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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- 710242 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GATA2 Recombinant Polyclonal Antibody (11HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with equine, non-human primate, mouse, rabbit and rat based on sequence homology.
- Antibody clone number
- 11HCLC
- Concentration
- 0.5 mg/mL
Submitted references 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB.
The flavonoid 4,4'-dimethoxychalcone promotes autophagy-dependent longevity across species.
Chen G, Xie W, Nah J, Sauvat A, Liu P, Pietrocola F, Sica V, Carmona-Gutierrez D, Zimmermann A, Pendl T, Tadic J, Bergmann M, Hofer SJ, Domuz L, Lachkar S, Markaki M, Tavernarakis N, Sadoshima J, Madeo F, Kepp O, Kroemer G
EMBO molecular medicine 2019 Nov 7;11(11):e10469
EMBO molecular medicine 2019 Nov 7;11(11):e10469
The flavonoid 4,4'-dimethoxychalcone promotes autophagy-dependent longevity across species.
Carmona-Gutierrez D, Zimmermann A, Kainz K, Pietrocola F, Chen G, Maglioni S, Schiavi A, Nah J, Mertel S, Beuschel CB, Castoldi F, Sica V, Trausinger G, Raml R, Sommer C, Schroeder S, Hofer SJ, Bauer MA, Pendl T, Tadic J, Dammbrueck C, Hu Z, Ruckenstuhl C, Eisenberg T, Durand S, Bossut N, Aprahamian F, Abdellatif M, Sedej S, Enot DP, Wolinski H, Dengjel J, Kepp O, Magnes C, Sinner F, Pieber TR, Sadoshima J, Ventura N, Sigrist SJ, Kroemer G, Madeo F
Nature communications 2019 Feb 19;10(1):651
Nature communications 2019 Feb 19;10(1):651
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA2 was performed by loading 30 µg of Rabbit Thyroid (lane1) and A431 (lane2) lysate using NuPAGE® Novex® 4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. GATA2 was detected at ~50 kDa using GATA2 Recombinant Rabbit Polyclonal Antibody (Product # 710242) at 2-3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA2 in K562 whole cell extracts using a GATA2 Recombinant Rabbit Polyclonal Antibody (Product # 710242) at a dilution of 2.5 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~50kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GATA2 in U2OS cells using a GATA2 Recombinant Rabbit Polyclonal Antibody (Product # 710242) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of GATA2.
