NBP1-82581

antibody from Novus Biologicals

Targeting: GATA2 NFE1B

Provider product page for NBP1-82581

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [4]

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Product number: NBP1-82581 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-82581, RRID:AB_11026191
Product name: Rabbit Polyclonal GATA-2 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human GATA-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

GATA2 protein structure - NBP1-82581 shown in red.

Submitted references The independent prognostic impact of the GATA2 pioneering factor is restricted to ERG-negative prostate cancer.

Büscheck F, Zub M, Heumann A, Hube-Magg C, Simon R, Lang DS, Höflmayer D, Neubauer E, Jacobsen F, Hinsch A, Luebke AM, Tsourlakis MC, Sauter G, Huland H, Graefen M, Haese A, Heinzer H, Schlomm T, Clauditz TS, Burandt E, Wilczak W, Steurer S, Minner S
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2019 Jul;41(7):1010428318824815

VE-Cadherin Is Required for Lymphatic Valve Formation and Maintenance.

Yang Y, Cha B, Motawe ZY, Srinivasan RS, Scallan JP
Cell reports 2019 Aug 27;28(9):2397-2412.e4

A blood capillary plexus-derived population of progenitor cells contributes to genesis of the dermal lymphatic vasculature during embryonic development.

Pichol-Thievend C, Betterman KL, Liu X, Ma W, Skoczylas R, Lesieur E, Bos FL, Schulte D, Schulte-Merker S, Hogan BM, Oliver G, Harvey NL, Francois M
Development (Cambridge, England) 2018 May 17;145(10)

Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program.

Frye M, Taddei A, Dierkes C, Martinez-Corral I, Fielden M, Ortsäter H, Kazenwadel J, Calado DP, Ostergaard P, Salminen M, He L, Harvey NL, Kiefer F, Mäkinen T
Nature communications 2018 Apr 17;9(1):1511

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Shi X, Richard J, Zirbes KM, Gong W, Lin G, Kyba M, Thomson JA, Koyano-Nakagawa N, Garry DJ
Developmental biology 2014 May 15;389(2):208-18

GATA2 as a potential metastasis-driving gene in prostate cancer.

Chiang YT, Wang K, Fazli L, Qi RZ, Gleave ME, Collins CC, Gout PW, Wang Y
Oncotarget 2014 Jan 30;5(2):451-61

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: GATA-2 Antibody [NBP1-82581] - Simple Western lane view shows a specific band for GATA2 in 0.2 mg/mL of HELA lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: GATA-2 Antibody [NBP1-82581] - Electropherogram image(s) of corresponding Simple Western lane view. GATA-2 antibody was used at 1:100 dilution on HELA lysate(s).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GATA-2 Antibody [NBP1-82581] - Analysis in human cell line SH-SY5Y.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: GATA-2 Antibody [NBP1-82581] - Analysis in control (vector only transfected HEK293T lysate) and GATA2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human kidney shows nuclear positivity in cells in tubules.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human placenta shows nuclear positivity in trophoblastic cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: GATA-2 Antibody [NBP1-82581] - Staining of human prostate shows nuclear positivity in glandular cells.