- MA5-24860 - Invitrogen Antibodies DGCR8 antibody

Submitted references Small RNA expression and miRNA modification dynamics in human oocytes and early embryos.

Paloviita P, Hydén-Granskog C, Yohannes DA, Paluoja P, Kere J, Tapanainen JS, Krjutškov K, Tuuri T, Võsa U, Vuoristo S
Genome research 2021 Aug;31(8):1474-1485

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with DGCR8 monoclonal antibody (Product # MA5-24860).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 in PC12 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with DGCR8 monoclonal antibody (Product # MA5-24860).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with DGCR8 monoclonal antibody (Product # MA5-24860).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DGCR8 Polyclonal Antibody (Product # MA5-24860) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of DGCR8 in HEK-293 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 in PC12 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with DGCR8 monoclonal antibody (Product # MA5-24860).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with DGCR8 monoclonal antibody (Product # MA5-24860).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of DGCR8 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DGCR8 Polyclonal Antibody (Product # MA5-24860) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of DGCR8 in HEK-293 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of DGCR8 in paraffin-embedded human colon carcinoma. Samples were incubated with DGCR8 polyclonal antibody (Product # MA5-24860) using a dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of DGCR8 in paraffin-embedded rat ovary. Samples were incubated with DGCR8 polyclonal antibody (Product # MA5-24860) using a dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. Small RNA (sRNA) pathway component expression in human oocytes and embryos. ( A ) Human four-cell ( left ) and eight-cell ( right ) embryos immunostained with DGCR8 (green) and DICER1 (magenta) antibodies. Nuclei are counterstained with DAPI (blue). Overlay of a single representative z -plane and the corresponding z -planes are shown. Nucleus and bright field channels are on the right side of each overlay. Scale bar is 50 mum. Expression levels (RPKM) in human oocytes and preimplantation embryos (): ( B ) miRNA pathway gene, DGCR8 , DROSHA , DICER1 , and AGO1-4 ; ( C ) piRNA pathway gene, PLD6 , HENMT1 , and PIWIL1-4 . ( B , C ) Significant expression changes between consecutive developmental stages were assessed using pairwise, two-sided Wilcoxon rank-sum tests: (*) FDR < 0.05; (**) FDR < 0.01; (***) FDR < 0.001. The horizontal line in the box plot indicates the expression median.

MA5-24860

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