10-7602-25

antibody from ABEOMICS Inc.

Targeting: VWF F8VWF

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

10-7602-25

Provider

ABEOMICS Inc.

Product name

Anti-VWF Antibody

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

A partial length recombinant protei
n (a.a 1710-1939) of VWF was used
as the immunogen for this antibody
.


Isotype

IgG

Antibody clone number

ABM53D2

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing

Western blot

Supportive validation

Submitted by

ABEOMICS Inc. (provider)

Main image

Experimental details

Western blot analysis of VWF. Anti-VWF antibody (Clone : ABM53D2) was used at 2 µg/ml on h Lungs lysate.

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

ABEOMICS Inc. (provider)

Main image

Experimental details

Intracellular flow analysis of VWF antibody on HepG2 cells using 0.5 µg/10^6 cells of antibody (Clone: ABM53D2). Green represents isotype control; red represents anti-VWF antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).

Protocol

Protocol