Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF2764 - Provider product page
- Provider
- R&D Systems
- Product name
- Human vWF-A2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human vWF-A2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_000543
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Coronary angiogenic effect of long-term administration of Nigella sativa.
Al Asoom LI
BMC complementary and alternative medicine 2017 Jun 13;17(1):308
BMC complementary and alternative medicine 2017 Jun 13;17(1):308
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human vWF-A2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human vWF-A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2764) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for vWF-A2 at approximately 250-460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.