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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Protein C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Protein C using 30 µg of A) HepG2 and B) HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Protein C polyclonal antibody (Product # PA5-28321) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Protein C was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Protein C Polyclonal Antibody (Product # PA5-28321) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Protein C in methanol-fixed HeLa cells using a Protein C polyclonal antibody (Product # PA5-28321) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using Protein C (Product # PA5-28321) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
