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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX108277 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108277, RRID:AB_1952233
- Product name
- Thrombomodulin antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: THP-1 7.5% SDS PAGE GTX108277 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Thrombomodulin antibody [C3], C-term detects Thrombomodulin protein at junction by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Thrombomodulin protein stained by Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Thrombomodulin protein from THP-1 whole cell extracts using 5 £gg of Thrombomodulin antibody [C3], C-term (GTX108277).Western blot analysis was performed using Thrombomodulin antibody [C3], C-term (GTX108277).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Thrombomodulin antibody [C3], C-term detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Thrombomodulin antibody [C3], C-term (GTX108277) dilution: 1:500.
