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ELISA
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB3947-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Thrombomodulin/BDCA-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Thrombomodulin/BDCA-3 in ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07204(Val473)- Isotype
- IgG
- Antibody clone number
- 501733
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, Adams Y
PloS one 2018;13(10):e0204177
PloS one 2018;13(10):e0204177
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Thrombomodulin/BDCA-3 in Human Kidney. Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Thrombomodulin/BDCA-3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human HLA-DR APC-conjugated Monoclonal Antibody (Catalog # FAB4869A) and either (A) Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
