Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF3894 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Thrombomodulin/BDCA-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Thrombomodulin/BDCA-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human Thrombomodulin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15306
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome.
Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia.
Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
Liu XY, Xu HX, Li JK, Zhang D, Ma XH, Huang LN, Lü JH, Wang XZ
Frontiers in physiology 2018;9:102
Frontiers in physiology 2018;9:102
Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia.
Nagy BM, Nagaraj C, Egemnazarov B, Kwapiszewska G, Stauber RE, Avian A, Olschewski H, Olschewski A
Frontiers in physiology 2017;8:98
Frontiers in physiology 2017;8:98
Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor.
Greineder CF, Chacko AM, Zaytsev S, Zern BJ, Carnemolla R, Hood ED, Han J, Ding BS, Esmon CT, Muzykantov VR
PloS one 2013;8(11):e80110
PloS one 2013;8(11):e80110
The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.
Schmidt EP, Yang Y, Janssen WJ, Gandjeva A, Perez MJ, Barthel L, Zemans RL, Bowman JC, Koyanagi DE, Yunt ZX, Smith LP, Cheng SS, Overdier KH, Thompson KR, Geraci MW, Douglas IS, Pearse DB, Tuder RM
Nature medicine 2012 Aug;18(8):1217-23
Nature medicine 2012 Aug;18(8):1217-23
Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
Graham BB, Mentink-Kane MM, El-Haddad H, Purnell S, Zhang L, Zaiman A, Redente EF, Riches DW, Hassoun PM, Bandeira A, Champion HC, Butrous G, Wynn TA, Tuder RM
The American journal of pathology 2010 Sep;177(3):1549-61
The American journal of pathology 2010 Sep;177(3):1549-61
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Thrombomodulin/BDCA-3 in bEnd.3 Mouse Cell Line. Thrombomodulin/BDCA-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Thrombomodulin/BDCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3894) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.