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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21924 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thrombomodulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: THP-1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Thrombomodulin/CD141 using 30 µg of THP-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Thrombomodulin/CD141 polyclonal antibody (Product # PA5-21924) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thrombomodulin antibody detects Thrombomodulin protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Thrombomodulin antibody Thrombomodulin Polyclonal Antibody (Product # PA5-21924) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Thrombomodulin was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombomodulin Polyclonal Antibody (Product # PA5-21924) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thrombomodulin Polyclonal Antibody detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Thrombomodulin Polyclonal Antibody (Product # PA5-21924) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
