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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ7756 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Thrombomodulin Antibody / THBD
- Antibody type
- Polyclonal
- Description
- This highly specific Thrombomodulin antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/Immunoprecipitation/Direct ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Thrombomodulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human RT4, 2) human A431 and 3) rat PC-12 cell lysate with Thrombomodulin antibody. Expected molecular weight: 60-100 kDa depending on glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with Thrombomodulin antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of Thrombomodulin protein from 500ug of human A431 whole cell lysate with 2ug of Thrombomodulin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with Thrombomodulin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen tissue with Thrombomodulin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human ThP-1 cells with Thrombomodulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thrombomodulin antibody.
