Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF137 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal P-Selectin/CD62P Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and rat P-Selectin/CD62P in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse P-Selectin, and less than 1% cross-reactivity with recombinant human (rh) E-Selectin and rhL-Selectin is observed.
- Reactivity
- Human, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies.
G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis.
Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy.
Stevenson NL, White IJ, McCormack JJ, Robinson C, Cutler DF, Nightingale TD
Journal of cell science 2017 Aug 1;130(15):2591-2605
Journal of cell science 2017 Aug 1;130(15):2591-2605
G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis.
Stevenson NL, Martin-Martin B, Freeman J, Kriston-Vizi J, Ketteler R, Cutler DF
Journal of thrombosis and haemostasis : JTH 2014 Feb;12(2):261-272
Journal of thrombosis and haemostasis : JTH 2014 Feb;12(2):261-272
Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin BV, Zarbock A, Gerke V, Rescher U
Nature communications 2014 Apr 28;5:3738
Nature communications 2014 Apr 28;5:3738
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Rauch C, Feifel E, Amann EM, Spötl HP, Schennach H, Pfaller W, Gstraunthaler G
ALTEX 2011;28(4):305-16
ALTEX 2011;28(4):305-16
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Rauch C, Feifel E, Amann EM, Spötl HP, Schennach H, Pfaller W, Gstraunthaler G
ALTEX 2011;28(4):305-16
ALTEX 2011;28(4):305-16
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, Sakamoto K, Matsuo S, Kadomatsu K
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy.
Choudhury A, Chung I, Blann AD, Lip GYH
Chest 2007 Mar;131(3):809-815
Chest 2007 Mar;131(3):809-815
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat P-Selectin/CD62P by Western Blot. Western blot shows lysates of rat platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P-Selectin/CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.