RQ4284

antibody from NSJ Bioreagents

Targeting: SELP CD62, CD62P, GMP140, GRMP, PADGEM, PSEL

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4284

Provider

NSJ Bioreagents

Product name

P-Selectin Antibody / CD62P

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the P-Selectin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse heart and 2) rat heart lysate with P-Selectin antibody. Expected molecular weight: 91-140 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse heart and 2) rat heart lysate with P-Selectin antibody. Expected molecular weight: 91-140 kDa depending on glycosylation level.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized mouse RAW264.7 cells with P-Selectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= P-Selectin antibody.