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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [5]
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- Product number
- NB100-65392 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65392, RRID:AB_960665
- Product name
- Mouse Monoclonal P-Selectin/CD62P Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
- Reactivity
- Human, Mouse, Rat, Bovine, Feline, Goat, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model.
Pharmacokinetics and Pharmacodynamics of an Oral Formulation of Apixaban in Horses After Oral and Intravenous Administration.
Subcutaneous Administration of Low-Molecular-Weight Heparin to Horses Inhibits Ex Vivo Equine Herpesvirus Type 1-Induced Platelet Activation.
Significant Hypo-Responsiveness to GPVI and CLEC-2 Agonists in Pre-Term and Full-Term Neonatal Platelets and following Immune Thrombocytopenia.
Endogenous fibrinolysis facilitates clot retraction in vivo.
Unfractionated and Low-Molecular-Weight Heparin and the Phosphodiesterase Inhibitors, IBMX and Cilostazol, Block Ex Vivo Equid Herpesvirus Type-1-Induced Platelet Activation.
Equid herpesvirus type 1 activates platelets.
Effects of clopidogrel on horses with experimentally induced endotoxemia.
Effects of clopidogrel on the platelet activation response in horses.
Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Characterization of platelet and soluble-porcine P-selectin (CD62P).
Characterization of platelet and soluble-porcine P-selectin (CD62P).
Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Horioka K, Tanaka H, Isozaki S, Okuda K, Asari M, Shiono H, Ogawa K, Shimizu K
Journal of thrombosis and haemostasis : JTH 2019 Oct;17(10):1762-1771
Journal of thrombosis and haemostasis : JTH 2019 Oct;17(10):1762-1771
Pharmacokinetics and Pharmacodynamics of an Oral Formulation of Apixaban in Horses After Oral and Intravenous Administration.
Serpa PBS, Brooks MB, Divers T, Ness S, Birschmann I, Papich MG, Stokol T
Frontiers in veterinary science 2018;5:304
Frontiers in veterinary science 2018;5:304
Subcutaneous Administration of Low-Molecular-Weight Heparin to Horses Inhibits Ex Vivo Equine Herpesvirus Type 1-Induced Platelet Activation.
Stokol T, Serpa PBS, Brooks MB, Divers T, Ness S
Frontiers in veterinary science 2018;5:106
Frontiers in veterinary science 2018;5:106
Significant Hypo-Responsiveness to GPVI and CLEC-2 Agonists in Pre-Term and Full-Term Neonatal Platelets and following Immune Thrombocytopenia.
Hardy AT, Palma-Barqueros V, Watson SK, Malcor JD, Eble JA, Gardiner EE, Blanco JE, Guijarro-Campillo R, Delgado JL, Lozano ML, Teruel-Montoya R, Vicente V, Watson SP, Rivera J, Ferrer-Marín F
Thrombosis and haemostasis 2018 Jun;118(6):1009-1020
Thrombosis and haemostasis 2018 Jun;118(6):1009-1020
Endogenous fibrinolysis facilitates clot retraction in vivo.
Samson AL, Alwis I, Maclean JAA, Priyananda P, Hawkett B, Schoenwaelder SM, Jackson SP
Blood 2017 Dec 7;130(23):2453-2462
Blood 2017 Dec 7;130(23):2453-2462
Unfractionated and Low-Molecular-Weight Heparin and the Phosphodiesterase Inhibitors, IBMX and Cilostazol, Block Ex Vivo Equid Herpesvirus Type-1-Induced Platelet Activation.
Stokol T, Serpa PBS, Zahid MN, Brooks MB
Frontiers in veterinary science 2016;3:99
Frontiers in veterinary science 2016;3:99
Equid herpesvirus type 1 activates platelets.
Stokol T, Yeo WM, Burnett D, DeAngelis N, Huang T, Osterrieder N, Catalfamo J
PloS one 2015;10(4):e0122640
PloS one 2015;10(4):e0122640
Effects of clopidogrel on horses with experimentally induced endotoxemia.
Watts AE, Ness SL, Divers TJ, Fubini SL, Frye AH, Stokol T, Cummings KJ, Brooks MB
American journal of veterinary research 2014 Aug;75(8):760-9
American journal of veterinary research 2014 Aug;75(8):760-9
Effects of clopidogrel on the platelet activation response in horses.
Brooks MB, Divers TJ, Watts AE, Ness SL, Frye AH, Stokol T, Fubini SL
American journal of veterinary research 2013 Sep;74(9):1212-22
American journal of veterinary research 2013 Sep;74(9):1212-22
Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
Shirasuna K, Jiemtaweeboon S, Raddatz S, Nitta A, Schuberth HJ, Bollwein H, Shimizu T, Miyamoto A
PloS one 2012;7(1):e29054
PloS one 2012;7(1):e29054
Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Johnson CA Jr, Wearden PD, Kocyildirim E, Maul TM, Woolley JR, Ye SH, Strickler EM, Borovetz HS, Wagner WR
Artificial organs 2011 Jun;35(6):602-13
Artificial organs 2011 Jun;35(6):602-13
Characterization of platelet and soluble-porcine P-selectin (CD62P).
Massaguer A, Engel P, Tovar V, March S, Rigol M, Solanes N, Bosch J, Pizcueta P
Veterinary immunology and immunopathology 2003 Dec 15;96(3-4):169-81
Veterinary immunology and immunopathology 2003 Dec 15;96(3-4):169-81
Characterization of platelet and soluble-porcine P-selectin (CD62P).
Massaguer A, Engel P, Tovar V, March S, Rigol M, Solanes N, Bosch J, Pizcueta P
Veterinary immunology and immunopathology 2003 Dec 15;96(3-4):169-81
Veterinary immunology and immunopathology 2003 Dec 15;96(3-4):169-81
Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Massaguer A, Engel P, Pérez-del-Pulgar S, Bosch J, Pizcueta P
Tissue antigens 2000 Aug;56(2):117-28
Tissue antigens 2000 Aug;56(2):117-28
Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Massaguer A, Engel P, Pérez-del-Pulgar S, Bosch J, Pizcueta P
Tissue antigens 2000 Aug;56(2):117-28
Tissue antigens 2000 Aug;56(2):117-28
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).
