- MA1-80910 - Invitrogen Antibodies ICAM1 antibody

Submitted references Neutrophils impose strong immune pressure against PfEMP1 variants implicated in cerebral malaria.

Zelter T, Strahilevitz J, Simantov K, Yajuk O, Adams Y, Ramstedt Jensen A, Dzikowski R, Granot Z
EMBO reports 2022 Jun 7;23(6):e53641

ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo.

Chong DLW, Rebeyrol C, José RJ, Williams AE, Brown JS, Scotton CJ, Porter JC
Frontiers in immunology 2021;12:691957

Use of thermodynamic coupling between antibody-antigen binding and phospholipid acyl chain phase transition energetics to predict immunoliposome targeting affinity.

Klegerman ME, Zou Y, Golunski E, Peng T, Huang SL, McPherson DD
Journal of liposome research 2014 Sep;24(3):216-22

Histopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta.

Hromatka BS, Ngeleza S, Adibi JJ, Niles RK, Tshefu AK, Fisher SJ
Biology of reproduction 2013 Jun;88(6):154

Nitric oxide improves molecular imaging of inflammatory atheroma using targeted echogenic immunoliposomes.

Kim H, Kee PH, Rim Y, Moody MR, Klegerman ME, Vela D, Huang SL, McPherson DD, Laing ST
Atherosclerosis 2013 Dec;231(2):252-60

Expression of intercellular adhesion molecule-1 in umbilical and placental vascular tissue of gestational diabetic and normal pregnancies.

Kurt M, Zulfikaroglu E, Ucankus NL, Omeroglu S, Ozcan U
Archives of gynecology and obstetrics 2010 Jan;281(1):71-6

Palm tissue displaying a polyclonal autoimmune response in patients affected by a new variant of endemic pemphigus foliaceus in Colombia, South America.

Abreu Velez AM, Howard MS, Hashimoto T
European journal of dermatology : EJD 2010 Jan-Feb;20(1):74-81

Novel anti-inflammatory properties of the angiogenesis inhibitor vasostatin.

Huegel R, Velasco P, De la Luz Sierra M, Christophers E, Schröder JM, Schwarz T, Tosato G, Lange-Asschenfeldt B
The Journal of investigative dermatology 2007 Jan;127(1):65-74

Intravascular ultrasound molecular imaging of atheroma components in vivo.

Hamilton AJ, Huang SL, Warnick D, Rabbat M, Kane B, Nagaraj A, Klegerman M, McPherson DD
Journal of the American College of Cardiology 2004 Feb 4;43(3):453-60

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of human peripheral blood monocytes using a ICAM-1/CD54 monoclonal antibody (Product # MA1-80910)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of human peripheral blood monocytes using a ICAM-1/CD54 monoclonal antibody (Product # MA1-80910)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of human peripheral blood monocytes using a ICAM-1/CD54 monoclonal antibody (Product # MA1-80910)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of human peripheral blood monocytes using a ICAM-1/CD54 monoclonal antibody (Product # MA1-80910)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells with1 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eBioscience™ (Product # 14-4714-82, yellow histogram) or1 µgICAM-1 Monoclonal Antibody (15.2) (Product # MA1-80910, blue histogram) followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A55058, 1:1000).Raji Cas9 control cells were also stained with1 µgICAM-1 Monoclonal Antibody (15.2) (Product # MA1-80910, pink histogram) followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488. Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone 15.2 but not in the control Cas9cells. Fixable Viability DyeeFluor780 (Product # 65-0865-18) was used for staining and selecting viable cells for analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: EV1 Figure The impact of phagocytosis on apoptosis and necrosis of PLB985 neutrophils A Annexin V and propidium iodide (PI) flow cytometry (FACS) were used to analyze for apoptosis and necrosis of GFP - neutrophils that did not phagocytose (left panel) or GFP + neutrophils that did phagocytose GFP-expressing P. falciparum iRBCs following a 6-h co-incubation. Neutrophils reduce the parasitemia in cultures. B Percentage of luciferase signal reduction during the short killing assay (6 h) of luciferase-expressing P. falciparum iRBCs with or without neutrophils. C iRBCs used for the short-term killing assay in (B) were put back into culture and the reduction in parasitemia was measured following 1 and 3 days of co-culture. D Percentage of parasitemia reduction on days 1 and 3 shown in B. E Reduction in parasitemia at day 1 and 3 following neutrophils incubation with iRBC in the presence or absence of catalase. The impact of serum proteins on the neutrophil killing of iRBCs. F Short-term killing assay of AB + -serum opsonized (AB + serum) and non-opsonized iRBCs (no serum) in the presence of anti-ICAM1 blocking antibody (mAb 15.2) and anti-CD11b control antibody. Data information: Results represent the average of three biological replicates ( n = 3) +- standard error of the mean (SEM). Statistical significance was determined using student t -test * P < 0.05; ** P < 0.01.

MA1-80910

Antibody data