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- Antibody Data
- Antigen structure
- References [38]
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- Product number
- AF796 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ICAM-1/CD54 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models.
Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke.
Early and Late Protective Effect of Bone Marrow Mononuclear Cell Transplantation on Radiation-Induced Vascular Dysfunction and Skin Lesions.
IgA1 deposition may induce NLRP3 expression and macrophage transdifferentiation of podocyte in IgA nephropathy.
Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells.
Complement activation contributes to perioperative neurocognitive disorders in mice.
Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice.
Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance, and Neurovascular Adhesion Molecule Expression.
Interferon-γ treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sjögren's Syndrome.
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis.
Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation.
Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway.
Epidermal RAF prevents allergic skin disease.
Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response.
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Peptide inhibitor of NF-κB translocation ameliorates experimental atherosclerosis.
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Lung-targeted overexpression of the NF-κB member RelB inhibits cigarette smoke-induced inflammation.
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency.
Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance.
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.
PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms.
Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
High pressure promotes monocyte adhesion to the vascular wall.
Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Riegler J, Gill H, Ogasawara A, Hedehus M, Javinal V, Oeh J, Ferl GZ, Marik J, Williams S, Sampath D, Schartner J, Carano RAD
Neoplasia (New York, N.Y.) 2019 Oct;21(10):1036-1050
Neoplasia (New York, N.Y.) 2019 Oct;21(10):1036-1050
Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke.
Salmeron KE, Maniskas ME, Edwards DN, Wong R, Rajkovic I, Trout A, Rahman AA, Hamilton S, Fraser JF, Pinteaux E, Bix GJ
Journal of neuroinflammation 2019 Nov 14;16(1):222
Journal of neuroinflammation 2019 Nov 14;16(1):222
Early and Late Protective Effect of Bone Marrow Mononuclear Cell Transplantation on Radiation-Induced Vascular Dysfunction and Skin Lesions.
Holler V, Buard V, Roque T, Squiban C, Benderitter M, Flamant S, Tamarat R
Cell transplantation 2019 Jan;28(1):116-128
Cell transplantation 2019 Jan;28(1):116-128
IgA1 deposition may induce NLRP3 expression and macrophage transdifferentiation of podocyte in IgA nephropathy.
Peng W, Pei GQ, Tang Y, Tan L, Qin W
Journal of translational medicine 2019 Dec 3;17(1):406
Journal of translational medicine 2019 Dec 3;17(1):406
Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells.
Wohlrab P, Soto-Gonzales L, Benesch T, Winter MP, Lang IM, Markstaller K, Tretter V, Klein KU
Frontiers in physiology 2018;9:1754
Frontiers in physiology 2018;9:1754
Complement activation contributes to perioperative neurocognitive disorders in mice.
Xiong C, Liu J, Lin D, Zhang J, Terrando N, Wu A
Journal of neuroinflammation 2018 Sep 4;15(1):254
Journal of neuroinflammation 2018 Sep 4;15(1):254
Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice.
Li Z, Luo T, Ning X, Xiong C, Wu A
Neuroscience letters 2018 Nov 20;687:189-195
Neuroscience letters 2018 Nov 20;687:189-195
Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance, and Neurovascular Adhesion Molecule Expression.
Niraula A, Wang Y, Godbout JP, Sheridan JF
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 28;38(9):2328-2340
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 28;38(9):2328-2340
Interferon-γ treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sjögren's Syndrome.
Meng Z, Klinngam W, Edman MC, Hamm-Alvarez SF
PloS one 2017;12(9):e0184781
PloS one 2017;12(9):e0184781
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis.
Xu Y, Xu S, Liu P, Koroleva M, Zhang S, Si S, Jin ZG
Journal of the American Heart Association 2017 Nov 30;6(12)
Journal of the American Heart Association 2017 Nov 30;6(12)
Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation.
Gupta KK, Donahue DL, Sandoval-Cooper MJ, Castellino FJ, Ploplis VA
Scientific reports 2017 Mar 23;7(1):365
Scientific reports 2017 Mar 23;7(1):365
Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway.
Wei CC, Kong YY, Li GQ, Guan YF, Wang P, Miao CY
Scientific reports 2017 Apr 6;7(1):717
Scientific reports 2017 Apr 6;7(1):717
Epidermal RAF prevents allergic skin disease.
Raguz J, Jeric I, Niault T, Nowacka JD, Kuzet SE, Rupp C, Fischer I, Biggi S, Borsello T, Baccarini M
eLife 2016 Jul 19;5
eLife 2016 Jul 19;5
Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
Gupta KK, Donahue DL, Sandoval-Cooper MJ, Castellino FJ, Ploplis VA
PloS one 2015;10(3):e0120728
PloS one 2015;10(3):e0120728
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Mathias D, Mitchel RE, Barclay M, Wyatt H, Bugden M, Priest ND, Whitman SC, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PloS one 2015;10(3):e0119661
PloS one 2015;10(3):e0119661
Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw TM, Boyce JA
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5061-8
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5061-8
Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
Goh Q, Dearth CL, Corbett JT, Pierre P, Chadee DN, Pizza FX
Experimental cell research 2015 Feb 15;331(2):292-308
Experimental cell research 2015 Feb 15;331(2):292-308
ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
Norton JE Jr, Lytle AG, Shen S, Tzvetkov EP, Dorfmeier CL, McGettigan JP
PloS one 2014;9(1):e87098
PloS one 2014;9(1):e87098
Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response.
Dearth CL, Goh Q, Marino JS, Cicinelli PA, Torres-Palsa MJ, Pierre P, Worth RG, Pizza FX
PloS one 2013;8(3):e58486
PloS one 2013;8(3):e58486
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Rampanelli E, Dessing MC, Claessen N, Teske GJ, Joosten SP, Pals ST, Leemans JC, Florquin S
PloS one 2013;8(12):e84479
PloS one 2013;8(12):e84479
Peptide inhibitor of NF-κB translocation ameliorates experimental atherosclerosis.
Mallavia B, Recio C, Oguiza A, Ortiz-Muñoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C
The American journal of pathology 2013 May;182(5):1910-21
The American journal of pathology 2013 May;182(5):1910-21
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomarker insights 2012;7:87-104
Biomarker insights 2012;7:87-104
Lung-targeted overexpression of the NF-κB member RelB inhibits cigarette smoke-induced inflammation.
McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
The American journal of pathology 2011 Jul;179(1):125-33
The American journal of pathology 2011 Jul;179(1):125-33
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
American journal of physiology. Heart and circulatory physiology 2009 May;296(5):H1329-35
American journal of physiology. Heart and circulatory physiology 2009 May;296(5):H1329-35
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Kato N, Yuzawa Y, Kosugi T, Hobo A, Sato W, Miwa Y, Sakamoto K, Matsuo S, Kadomatsu K
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1565-76
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
Kidney international 2009 Jan;75(1):52-9
Kidney international 2009 Jan;75(1):52-9
Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
European journal of immunology 2009 Jan;39(1):85-95
European journal of immunology 2009 Jan;39(1):85-95
Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency.
Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K
Journal of cellular physiology 2009 Aug;220(2):508-14
Journal of cellular physiology 2009 Aug;220(2):508-14
Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance.
Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW
Arteriosclerosis, thrombosis, and vascular biology 2008 Nov;28(11):1982-8
Arteriosclerosis, thrombosis, and vascular biology 2008 Nov;28(11):1982-8
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
Circulation 2008 Jan 22;117(3):421-8
Circulation 2008 Jan 22;117(3):421-8
Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.
Casós K, Sáiz MP, Ruiz-Sanz JI, Mitjavila MT
Atherosclerosis 2008 Dec;201(2):306-17
Atherosclerosis 2008 Dec;201(2):306-17
PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms.
Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R
Brain research 2008 Dec 9;1244:164-72
Brain research 2008 Dec 9;1244:164-72
Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC
Circulation research 2007 Nov 9;101(10):1001-8
Circulation research 2007 Nov 9;101(10):1001-8
Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Förster I, Huss R, Stratis A, Ruzicka T, Röcken M, Pfeffer K, Schmid RM, Rupec RA
Immunity 2007 Aug;27(2):296-307
Immunity 2007 Aug;27(2):296-307
High pressure promotes monocyte adhesion to the vascular wall.
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circulation research 2007 Apr 27;100(8):1226-33
Circulation research 2007 Apr 27;100(8):1226-33
Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.
Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA
British journal of pharmacology 2006 Nov;149(5):591-9
British journal of pharmacology 2006 Nov;149(5):591-9
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R 3rd
American journal of physiology. Lung cellular and molecular physiology 2006 May;290(5):L962-70
American journal of physiology. Lung cellular and molecular physiology 2006 May;290(5):L962-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ICAM-1/CD54 in Mouse Testis. ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
