AF720

antibody from Novus Biologicals

Targeting: ICAM1 BB2, CD54

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF720

Provider

Novus Biologicals

Product name

Sheep Polyclonal ICAM-1/CD54 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Selected for its ability to block the adhesion of HSB-2 cells to immobilized recombinant human (rh) ICAM-1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM-1, recombinant mouse ICAM-1, and rhVCAM-1 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction.

Genshaft AS, Li S, Gallant CJ, Darmanis S, Prakadan SM, Ziegler CG, Lundberg M, Fredriksson S, Hong J, Regev A, Livak KJ, Landegren U, Shalek AK
Genome biology 2016 Sep 19;17(1):188

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High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.

Hashizume M, Mihara M
Biochemical and biophysical research communications 2010 Dec 10;403(2):184-9

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C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.

Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
Journal of leukocyte biology 2008 Oct;84(4):1109-19

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Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.

Schellerer VS, Croner RS, Weinländer K, Hohenberger W, Stürzl M, Naschberger E
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1159-70

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The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.

Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis and rheumatism 2007 Jun;56(6):1817-26

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Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.

Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R
Blood 2007 Feb 15;109(4):1381-6

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human ICAM-1/CD54 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA) for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.