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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB583 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ICAM-1/CD54 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) ICAM-1, rmICAM-2, recombinant human (rh) ICAM-2, rhICAM-3, rmVCAM-1, or rhPCAM-1.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
Cell-based angiopoietin-1 gene therapy for acute lung injury.
Li PC, Chen WC, Chang LC, Lin SC
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L912-20
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L912-20
Cell-based angiopoietin-1 gene therapy for acute lung injury.
McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
American journal of respiratory and critical care medicine 2007 May 15;175(10):1014-26
American journal of respiratory and critical care medicine 2007 May 15;175(10):1014-26
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Rat ICAM-1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB583) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
