Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF583 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat ICAM-1/CD54 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM-3, and rmICAM-2 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q00238
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Effects of the Emitted Light Spectrum of Liquid Crystal Displays on Light-Induced Retinal Photoreceptor Cell Damage.
Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis.
Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation.
Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.
Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice.
Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Sustained elevation of NF-κB activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1α recovery.
Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
Lin CW, Yang CM, Yang CH
International journal of molecular sciences 2019 May 10;20(9)
International journal of molecular sciences 2019 May 10;20(9)
Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis.
Huang J, Han S, Sun Q, Zhao Y, Liu J, Yuan X, Mao W, Peng B, Liu W, Yin J, He X
Cell & bioscience 2017;7:31
Cell & bioscience 2017;7:31
Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation.
Kovacs ZI, Kim S, Jikaria N, Qureshi F, Milo B, Lewis BK, Bresler M, Burks SR, Frank JA
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 3;114(1):E75-E84
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 3;114(1):E75-E84
Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.
Hu G, Wang J, Hong D, Zhang T, Duan H, Mu X, Yang Z
BMC complementary and alternative medicine 2017 Jan 11;17(1):38
BMC complementary and alternative medicine 2017 Jan 11;17(1):38
Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice.
Bloomer SA, Wellen KE, Henderson GC
Lipids in health and disease 2017 Dec 13;16(1):243
Lipids in health and disease 2017 Dec 13;16(1):243
Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Yeh PT, Huang HW, Yang CM, Yang WS, Yang CH
PloS one 2016;11(1):e0146438
PloS one 2016;11(1):e0146438
Sustained elevation of NF-κB activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1α recovery.
Deng Y, Zhang Q, Luo H, Chen X, Han Q, Wang F, Huang P, Lai W, Guan X, Pan X, Ji Y, Guo W, Che L, Tang Y, Gu L, Yu J, Namaka M, Deng Y, Li X
Scientific reports 2016 Sep 12;6:32642
Scientific reports 2016 Sep 12;6:32642
Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
Hanusch C, Nowak K, Gill IS, Törlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
Clinical and experimental immunology 2007 Aug;149(2):364-71
Clinical and experimental immunology 2007 Aug;149(2):364-71
Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
Diabetes 2005 Dec;54(12):3418-26
Diabetes 2005 Dec;54(12):3418-26
Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
Diabetes 2005 Dec;54(12):3418-26
Diabetes 2005 Dec;54(12):3418-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat ICAM-1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ICAM-1/CD54 in Rat Brain. ICAM-1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.