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- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Flow cytometry [3]
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- Product number
- NB500-318 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-318, RRID:AB_10002731
- Product name
- Mouse Monoclonal ICAM-1/CD54 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury.
Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes.
Paclitaxel-induced lung injury and its amelioration by parecoxib sodium.
Inhibitory effects of suppressor of cytokine signaling 3 on inflammatory cytokine expression and migration and proliferation of IL-6/IFN-γ-induced vascular smooth muscle cells.
The role of repeatedly heated soybean oil in the development of hypertension in rats: association with vascular inflammation.
A physiologically realistic in vitro model of microvascular networks.
Carlos CP, Silva AA, Gil CD, Oliani SM
Scientific reports 2018 Jun 22;8(1):9568
Scientific reports 2018 Jun 22;8(1):9568
Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes.
Thomas A, Daniel Ou-Yang H, Lowe-Krentz L, Muzykantov VR, Liu Y
Biomicrofluidics 2016 Jan;10(1):014101
Biomicrofluidics 2016 Jan;10(1):014101
Paclitaxel-induced lung injury and its amelioration by parecoxib sodium.
Liu WJ, Zhong ZJ, Cao LH, Li HT, Zhang TH, Lin WQ
Scientific reports 2015 Aug 10;5:12977
Scientific reports 2015 Aug 10;5:12977
Inhibitory effects of suppressor of cytokine signaling 3 on inflammatory cytokine expression and migration and proliferation of IL-6/IFN-γ-induced vascular smooth muscle cells.
Xiang S, Dong NG, Liu JP, Wang Y, Shi JW, Wei ZJ, Hu XJ, Gong L
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2013 Oct;33(5):615-622
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2013 Oct;33(5):615-622
The role of repeatedly heated soybean oil in the development of hypertension in rats: association with vascular inflammation.
Ng CY, Kamisah Y, Faizah O, Jaarin K
International journal of experimental pathology 2012 Oct;93(5):377-87
International journal of experimental pathology 2012 Oct;93(5):377-87
A physiologically realistic in vitro model of microvascular networks.
Rosano JM, Tousi N, Scott RC, Krynska B, Rizzo V, Prabhakarpandian B, Pant K, Sundaram S, Kiani MF
Biomedical microdevices 2009 Oct;11(5):1051-7
Biomedical microdevices 2009 Oct;11(5):1051-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep12977), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE
