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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00003383-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003383-M01, RRID:AB_425498
- Product name
- ICAM1 monoclonal antibody (M01), clone 3H8-2G6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ICAM1.
- Antigen sequence
MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSK
VILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELL
LPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKT
FLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGG
APRANLTVVLLRGEKELKREPAVGEPAEVTTTVLV
RRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQ
TFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPV
SEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTA
EDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNV
ILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPL
GPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKN
QTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQ
AWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTY
LRRARSTQGEVTRKVAVNVLSPRYEIVIITVVAAA
VIMGTAGLSTYLYNRQRKIKKYRLQQAQKGTPMKP
NTQATPP- Isotype
- IgG
- Antibody clone number
- 3H8-2G6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Lipid-induced toxicity stimulates hepatocytes to release angiogenic microparticles that require Vanin-1 for uptake by endothelial cells.
Povero D, Eguchi A, Niesman IR, Andronikou N, de Mollerat du Jeu X, Mulya A, Berk M, Lazic M, Thapaliya S, Parola M, Patel HH, Feldstein AE
Science signaling 2013 Oct 8;6(296):ra88
Science signaling 2013 Oct 8;6(296):ra88
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ICAM1 expression in transfected 293T cell line by ICAM1 monoclonal antibody (M01), clone 3H8-2G6.Lane 1: ICAM1 transfected lysate (Predicted MW: 57.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ICAM1 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ICAM1 transfected lysate using anti-ICAM1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ICAM1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
