- MHCD5401 - Invitrogen Antibodies ICAM1 antibody

Submitted references High phosphate induces a pro-inflammatory response by vascular smooth muscle cells and modulation by vitamin D derivatives.

Martínez-Moreno JM, Herencia C, de Oca AM, Díaz-Tocados JM, Vergara N, Gómez-Luna MJ, López-Argüello SD, Camargo A, Peralbo-Santaella E, Rodríguez-Ortiz ME, Canalejo A, Rodríguez M, Muñoz-Castañeda JR, Almadén Y
Clinical science (London, England : 1979) 2017 Jul 1;131(13):1449-1463

Low-dose acetaminophen induces early disruption of cell-cell tight junctions in human hepatic cells and mouse liver.

Gamal W, Treskes P, Samuel K, Sullivan GJ, Siller R, Srsen V, Morgan K, Bryans A, Kozlowska A, Koulovasilopoulos A, Underwood I, Smith S, Del-Pozo J, Moss S, Thompson AI, Henderson NC, Hayes PC, Plevris JN, Bagnaninchi PO, Nelson LJ
Scientific reports 2017 Jan 30;7:37541

Aging-associated oxidized albumin promotes cellular senescence and endothelial damage.

Luna C, Alique M, Navalmoral E, Noci MV, Bohorquez-Magro L, Carracedo J, Ramírez R
Clinical interventions in aging 2016;11:225-36

TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity.

Luna C, Carmona A, Alique M, Carracedo J, Ramirez R
Frontiers in physiology 2015;6:395

Effect of intravenous coadministration of human stroma cell lines on engraftment of long-term repopulating clonal myelodysplastic syndrome cells in immunodeficient mice.

Li X, Marcondes AM, Ragoczy T, Telling A, Deeg HJ
Blood cancer journal 2013 Apr 26;3(4):e113

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Localization and colocalization of human HS27a stroma and hematopoietic cells in murine marrow (BM) and spleen. ( a ) Confocal microscopy showing colocalization of KG1a cells and HS27a stroma in fresh frozen sections of spleen (original magnification: x 40): HS27a cells are labeled with FITC-conjugated anti-human ICAM1 antibody. Red indicates human CD45+ KG1a cells, whereas blue shows nuclear staining with DAPI (both murine and human nuclei stain with DAPI). The right lower panel represents the merged picture. Superimposition of the FITC signals of the anti-ICAM1 antibody and the Alexa 647 signals of the CD45 antibody results in a yellow hue of signals. White arrows indicate examples of staining for colocalizing HS27a stroma and CD45+ cells. ( b ) Spleen sections (formalin fixed) stained with anti-human ICAM1 antibody (green) and anti-CD45 antibody (red), merged in the right panel (original magnification= x 40). The lower panels show isotype controls. ( c ) Immunohistochemical determination of the distribution of primary MDS cells (labeled with anti-human (h) CD34 and CD45 antibodies) and HS27a stroma (labeled with anti-human ICAM1 antibody) in the bone marrow and spleen of NSG mice. White arrows indicate identical coordinates on sequential sections (section distance=4 mum; orignal magnification= x 40). ( d ) Immunohistochemical staining of formalin-fixed spleen sections labeled with anti-human CD146 antibody (original magnification= x 40). Dark brown identifies 3,3&
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Flow cytometer analysis of endothelial adhesion molecules, ( A ) VCAM-1 and ( B ) ICAM-1, expression in HUVEC incubated with vehicle CuSO 4 (10 umol/L) + 0.1 mmol/L EDTA (gray bar), Nat Alb (white bars), or Ox Alb (black bars). Notes: HUVEC were treated with different doses (mg/mL) of native and oxidized albumin for 24 hours. MFI are presented as mean +- SD, n=3, in duplicate. * P
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 (A) ICAM expression obtained by flow cytometry of HUVECs treated with 10 ng/ml TNFalpha at different time points. The expression is quantified by changes in the mean fluorescent channel (MFC). (B) PECAM expression obtained by flow cytometry on HUVECS treated with 10 ng/ml TNFalpha at different time points. Expression units are similar to ICAM (MFC). (C) Differences in total numbers of annexin V+/IP+ cells (late apoptosis) observed via flow cytometry in cultures treated with TNFalpha at different time points. These values are compared with the control culture, in which the number of apoptotic cells is set to 1. (D) Decrease in the number of PCNA+ cells (proliferating cells) in cultures treated with TNFalpha at different time points. * vs. control, # vs. TNFalpha 24 h, SS vs. TNFalpha 48 h. ** p < 0.05; *** p < 0.001.
Conjugate: Green dye

MHCD5401

Antibody data