Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [4]
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- Product number
- 12-0549-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD54 (ICAM-1) Monoclonal Antibody (HA58), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response. Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HA58 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- HA58
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells.
Impact of latency-reversing agents on human macrophage physiology.
Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs).
Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.
Potential Suppressive Effect of Nicotine on the Inflammatory Response in Oral Epithelial Cells: An In Vitro Study.
Increased Production of LIGHT by T Cells in Eosinophilic Esophagitis Promotes Differentiation of Esophageal Fibroblasts Toward an Inflammatory Phenotype.
DRM02, a novel phosphodiesterase-4 inhibitor with cutaneous anti-inflammatory activity.
Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients.
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.
Expansion of functional personalized cells with specific transgene combinations.
Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.
CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.
The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.
Trafficking of adeno-associated virus vectors across a model of the blood-brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells.
Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
Activation of fibroblast-like synoviocytes derived from rheumatoid arthritis via lysophosphatidic acid-lysophosphatidic acid receptor 1 cascade.
Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.
Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells.
Minimal encounter time and separation determine ligand-receptor binding in cell adhesion.
Bortezomib overcomes cell-adhesion-mediated drug resistance through downregulation of VLA-4 expression in multiple myeloma.
Characterization of fibroblasts responsible for cartilage destruction in arthritis.
Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis.
Requirements for ICAM-1 immunogene therapy of lymphoma.
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
The human natural killer cell immune synapse.
Wünnemann F, Fotsing Tadjo T, Beaudoin M, Lalonde S, Lo KS, Kleinstiver BP, Lettre G
PLoS genetics 2023 Mar;19(3):e1010680
PLoS genetics 2023 Mar;19(3):e1010680
Impact of latency-reversing agents on human macrophage physiology.
Hany L, Turmel MO, Barat C, Ouellet M, Tremblay MJ
Immunity, inflammation and disease 2023 Jan;11(1):e590
Immunity, inflammation and disease 2023 Jan;11(1):e590
Anti-Inflammatory Activity of Ferula assafoetida Oleo-Gum-Resin (Asafoetida) against TNF-α-Stimulated Human Umbilical Vein Endothelial Cells (HUVECs).
Mobasheri L, Khorashadizadeh M, Safarpour H, Mohammadi M, Anani Sarab G, Askari VR
Mediators of inflammation 2022;2022:5171525
Mediators of inflammation 2022;2022:5171525
Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2.
Shrestha P, Davis DA, Jaeger HK, Stream A, Aisabor AI, Yarchoan R
PLoS pathogens 2021 Jan;17(1):e1009091
PLoS pathogens 2021 Jan;17(1):e1009091
Potential Suppressive Effect of Nicotine on the Inflammatory Response in Oral Epithelial Cells: An In Vitro Study.
An N, Holl J, Wang X, Rausch MA, Andrukhov O, Rausch-Fan X
International journal of environmental research and public health 2021 Jan 9;18(2)
International journal of environmental research and public health 2021 Jan 9;18(2)
Increased Production of LIGHT by T Cells in Eosinophilic Esophagitis Promotes Differentiation of Esophageal Fibroblasts Toward an Inflammatory Phenotype.
Manresa MC, Chiang AWT, Kurten RC, Dohil R, Brickner H, Dohil L, Herro R, Akuthota P, Lewis NE, Croft M, Aceves SS
Gastroenterology 2020 Nov;159(5):1778-1792.e13
Gastroenterology 2020 Nov;159(5):1778-1792.e13
DRM02, a novel phosphodiesterase-4 inhibitor with cutaneous anti-inflammatory activity.
Hunt DWC, Ivanova IA, Dagnino L
Tissue barriers 2020 Jul 2;8(3):1765633
Tissue barriers 2020 Jul 2;8(3):1765633
Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients.
Lim SA, Kim J, Jeon S, Shin MH, Kwon J, Kim TJ, Im K, Han Y, Kwon W, Kim SW, Yee C, Kim SJ, Jang JY, Lee KM
Frontiers in immunology 2019;10:496
Frontiers in immunology 2019;10:496
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.
Louveau A, Herz J, Alme MN, Salvador AF, Dong MQ, Viar KE, Herod SG, Knopp J, Setliff JC, Lupi AL, Da Mesquita S, Frost EL, Gaultier A, Harris TH, Cao R, Hu S, Lukens JR, Smirnov I, Overall CC, Oliver G, Kipnis J
Nature neuroscience 2018 Oct;21(10):1380-1391
Nature neuroscience 2018 Oct;21(10):1380-1391
Expansion of functional personalized cells with specific transgene combinations.
Lipps C, Klein F, Wahlicht T, Seiffert V, Butueva M, Zauers J, Truschel T, Luckner M, Köster M, MacLeod R, Pezoldt J, Hühn J, Yuan Q, Müller PP, Kempf H, Zweigerdt R, Dittrich-Breiholz O, Pufe T, Beckmann R, Drescher W, Riancho J, Sañudo C, Korff T, Opalka B, Rebmann V, Göthert JR, Alves PM, Ott M, Schucht R, Hauser H, Wirth D, May T
Nature communications 2018 Mar 8;9(1):994
Nature communications 2018 Mar 8;9(1):994
Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.
Hough KP, Wilson LS, Trevor JL, Strenkowski JG, Maina N, Kim YI, Spell ML, Wang Y, Chanda D, Dager JR, Sharma NS, Curtiss M, Antony VB, Dransfield MT, Chaplin DD, Steele C, Barnes S, Duncan SR, Prasain JK, Thannickal VJ, Deshane JS
Scientific reports 2018 Jul 9;8(1):10340
Scientific reports 2018 Jul 9;8(1):10340
CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.
Li C, Liu S, Yan R, Han N, Wong KK, Li L
Theranostics 2017;7(1):67-80
Theranostics 2017;7(1):67-80
The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.
Qiu C, Wang Y, Zhao H, Qin L, Shi Y, Zhu X, Song L, Zhou X, Chen J, Zhou H, Zhang H, Tellides G, Min W, Yu L
Nature communications 2017 Jun 1;8:15426
Nature communications 2017 Jun 1;8:15426
Trafficking of adeno-associated virus vectors across a model of the blood-brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells.
Merkel SF, Andrews AM, Lutton EM, Mu D, Hudry E, Hyman BT, Maguire CA, Ramirez SH
Journal of neurochemistry 2017 Jan;140(2):216-230
Journal of neurochemistry 2017 Jan;140(2):216-230
Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.
Faes S, Uldry E, Planche A, Santoro T, Pythoud C, Demartines N, Dormond O
Oncotarget 2016 Dec 27;7(52):86026-86038
Oncotarget 2016 Dec 27;7(52):86026-86038
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
An N, Andrukhov O, Tang Y, Falkensammer F, Bantleon HP, Ouyang X, Rausch-Fan X
PloS one 2014;9(5):e96942
PloS one 2014;9(5):e96942
Activation of fibroblast-like synoviocytes derived from rheumatoid arthritis via lysophosphatidic acid-lysophosphatidic acid receptor 1 cascade.
Miyabe Y, Miyabe C, Iwai Y, Yokoyama W, Sekine C, Sugimoto K, Harigai M, Miyasaka M, Miyasaka N, Nanki T
Arthritis research & therapy 2014 Oct 2;16(5):461
Arthritis research & therapy 2014 Oct 2;16(5):461
Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.
Liu S, Li N, Yu X, Xiao X, Cheng K, Hu J, Wang J, Zhang D, Cheng S, Liu S
Gastroenterology 2013 May;144(5):1031-1041.e10
Gastroenterology 2013 May;144(5):1031-1041.e10
Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells.
Qin Y, Li Y, Liu W, Tian R, Guo Q, Li S, Li H, Zhang D, Zheng Y, Wu L, Lan K, Wang J
PloS one 2011;6(11):e27609
PloS one 2011;6(11):e27609
Minimal encounter time and separation determine ligand-receptor binding in cell adhesion.
Robert P, Nicolas A, Aranda-Espinoza S, Bongrand P, Limozin L
Biophysical journal 2011 Jun 8;100(11):2642-51
Biophysical journal 2011 Jun 8;100(11):2642-51
Bortezomib overcomes cell-adhesion-mediated drug resistance through downregulation of VLA-4 expression in multiple myeloma.
Noborio-Hatano K, Kikuchi J, Takatoku M, Shimizu R, Wada T, Ueda M, Nobuyoshi M, Oh I, Sato K, Suzuki T, Ozaki K, Mori M, Nagai T, Muroi K, Kano Y, Furukawa Y, Ozawa K
Oncogene 2009 Jan 15;28(2):231-42
Oncogene 2009 Jan 15;28(2):231-42
Characterization of fibroblasts responsible for cartilage destruction in arthritis.
Treese C, Mittag A, Lange F, Tarnok A, Loesche A, Emmrich F, Lehmann J, Sack U
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2008 Apr;73(4):351-60
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2008 Apr;73(4):351-60
Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis.
Mandron M, Ariès MF, Boralevi F, Martin H, Charveron M, Taieb A, Davrinche C
The Journal of investigative dermatology 2008 Apr;128(4):882-9
The Journal of investigative dermatology 2008 Apr;128(4):882-9
Requirements for ICAM-1 immunogene therapy of lymphoma.
Kanwar JR, Berg RW, Yang Y, Kanwar RK, Ching LM, Sun X, Krissansen GW
Cancer gene therapy 2003 Jun;10(6):468-76
Cancer gene therapy 2003 Jun;10(6):468-76
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
Bao J, Sato K, Li M, Gao Y, Abid R, Aird W, Simons M, Post MJ
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
Bao J, Sato K, Li M, Gao Y, Abid R, Aird W, Simons M, Post MJ
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
The human natural killer cell immune synapse.
Davis DM, Chiu I, Fassett M, Cohen GB, Mandelboim O, Strominger JL
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD54 (ICAM-1) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells with0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE,eBioscience™ (Product # 12-4714-82, yellow histogram) or0.25 µgCD19 Monoclonal Antibody (HIB19), PE,eBioscience™ (Product # 12-0549-42, blue histogram).Raji Cas9 control cells were also stained with0.25 µgCD19 Monoclonal Antibody (HIB19), PE,eBioscience™ (Product # 12-0549-42, pink histogram). Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone HA58 but not in the control Cas9cells. Fixable Viability DyeeFluor780 (Product # 65-0865-18) was used for staining and selecting viable cells for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Loss of endothelial SENP1 inhibits EC activation. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 mum. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 and P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1beta treatment. Representative histograms are shown in ( b ) with the quantification of mean intensity in ( c - e ). ( f - h ) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines and analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 and VCAM-1 are shown in ( f ) with the quantification of mean intensity in ( g , h ). Data are presented as the mean+-s.e.m. from at least three independent experiments. * P
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Effect of nicotine on the P. gingivalis LPS-induced protein expression of pro-inflammatory mediators in HUVECs. HUVECs were stimulated by P. gingivalis LPS in the presence or absence of nicotine (10 uM-10 mM) for 4, 24, and 72 h. After stimulation, the surface expression levels of ICAM-1 (A), VCAM-1 (B), and E-selectin (C) were measured by flow cytometry, and the quantity of MCP-1 (D) and IL-8 (E) in conditioned media was measured by ELISA. Each value represents mean +-SD of three independent assays. Non-stimulated HUVECs were used as a control. The protein expression levels of pro-inflammatory mediators were not analyzed after stimulation with 10-mM nicotine for 24 and 72 h because the cells were not viable. * - significantly different between groups, p
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Cancer Stem Cell Traits of Differential Populations of CD54 + and CD54 - Cells. ( a ) FACS histograms show separate gating for the isolation of CD54 + and CD54 - PC3 and LNCaP cells. ( b ) Representative light micrograph fields and comparative quantification of the sphere-forming capacity of CD54 + and CD54 - LNCaP and PC3 cells. ( c ) Representative microscope fields from colony formation assays for CD54 + and CD54 - PC3 cells. ( d ) The cisplatin (DDP) IC 50 of CD54 + and CD54 - cells from the PC3, LNCaP, DU145, and 22RV1 prostate cancer cell lines. ( e ) q-PCR analysis of the expression of Hedgehog, Notch, and Wnt signaling pathway genes as well as genes involved in stem cell self-renewal in CD54 + and CD54 - PC3 cells. ( f ) Representative micrographs of in situ protein expression of the key signaling pathway components HES1 and CD54 in DAPI-stained LNCaP and PC3 cells. ** P < 0.01.
- Conjugate
- Yellow dye