Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-0549-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD54 (ICAM-1) Monoclonal Antibody (HA58), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response. Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- HA58
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer.
The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
Requirements for ICAM-1 immunogene therapy of lymphoma.
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
The human natural killer cell immune synapse.
The human natural killer cell immune synapse.
Li C, Liu S, Yan R, Han N, Wong KK, Li L
Theranostics 2017;7(1):67-80
Theranostics 2017;7(1):67-80
The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.
Qiu C, Wang Y, Zhao H, Qin L, Shi Y, Zhu X, Song L, Zhou X, Chen J, Zhou H, Zhang H, Tellides G, Min W, Yu L
Nature communications 2017 Jun 1;8:15426
Nature communications 2017 Jun 1;8:15426
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
An N, Andrukhov O, Tang Y, Falkensammer F, Bantleon HP, Ouyang X, Rausch-Fan X
PloS one 2014;9(5):e96942
PloS one 2014;9(5):e96942
Requirements for ICAM-1 immunogene therapy of lymphoma.
Kanwar JR, Berg RW, Yang Y, Kanwar RK, Ching LM, Sun X, Krissansen GW
Cancer gene therapy 2003 Jun;10(6):468-76
Cancer gene therapy 2003 Jun;10(6):468-76
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
Bao J, Sato K, Li M, Gao Y, Abid R, Aird W, Simons M, Post MJ
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation.
Bao J, Sato K, Li M, Gao Y, Abid R, Aird W, Simons M, Post MJ
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
American journal of physiology. Heart and circulatory physiology 2001 Dec;281(6):H2612-8
The human natural killer cell immune synapse.
Davis DM, Chiu I, Fassett M, Cohen GB, Mandelboim O, Strominger JL
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
The human natural killer cell immune synapse.
Davis DM, Chiu I, Fassett M, Cohen GB, Mandelboim O, Strominger JL
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
Proceedings of the National Academy of Sciences of the United States of America 1999 Dec 21;96(26):15062-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, Biotin (Product # 13-4714-85) (blue histogram) or CD54 (ICAM-1) Monoclonal Antibody, Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin