A00171

antibody from Boster Biological Technology

Targeting: ICAM1 BB2, CD54

Provider product page for A00171

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [33]
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Validations
Western blot [1]

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Product number: A00171 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-ICAM1 Antibody Picoband™
Antibody type: Polyclonal
Description: Polyclonal antibody for CD54/ICAM1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. CD54/ICAM1 information: Subcellular Localization: Membrane; Single-pass type I membrane protein.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ICAM1 protein structure - A00171 shown in red.

Submitted references 17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats.

Vidal-Dos-Santos M, Anunciação LF, Armstrong-Jr R, Ricardo-da-Silva FY, Ramos IYT, Correia CJ, Moreira LFP, Leuvenink HGD, Breithaupt-Faloppa AC
Frontiers in immunology 2024;15:1375943

Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm.

Hao L, Ya X, Wu J, Tao C, Ma R, Zheng Z, Mou S, Ling Y, Yang Y, Wang J, Zhang Y, Lin Q, Zhao J
Journal of biomedical science 2024 May 13;31(1):51

Differential vascular endothelial cell toxicity of established and novel BCR-ABL tyrosine kinase inhibitors.

Wang Y, Travers RJ, Farrell A, Lu Q, Bays JL, Stepanian A, Chen C, Jaffe IZ
PloS one 2023;18(11):e0294438

Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats.

de Sousa MN, da Anunciação LF, de Freitas PLZ, Ricardo-da-Silva FY, Moreira LFP, Correia CJ, Breithaupt-Faloppa AC
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2023 Sep 7;64(3)

GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation.

Xie S, Su E, Song X, Xue J, Yu P, Zhang B, Liu M, Jiang H
Biomedicines 2023 Sep 20;11(9)

Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy.

Zhang H, Jia S, Jin L, Mb JY, Shen Z, Wu J, Yao X, Chen D, Zhang C, Yu S, Zhu N, Jin L, Yao X
Acta cirurgica brasileira 2022;36(11):e361104

Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes.

Luo F, Li R, Zheng H, Xu Y, Yang L, Qu C, Hong G, Wan Q
Frontiers in bioengineering and biotechnology 2022;10:913080

Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy.

Lian Y, Zhu M, Chen J, Yang B, Lv Q, Wang L, Guo S, Tan X, Li C, Bu W, Ding W, Jia X, Feng L
International journal of biological macromolecules 2021 Apr 15;176:589-600

The Increased Endogenous Sulfur Dioxide Acts as a Compensatory Mechanism for the Downregulated Endogenous Hydrogen Sulfide Pathway in the Endothelial Cell Inflammation.

Zhang D, Wang X, Tian X, Zhang L, Yang G, Tao Y, Liang C, Li K, Yu X, Tang X, Tang C, Zhou J, Kong W, Du J, Huang Y, Jin H
Frontiers in immunology 2018;9:882

Oxidized low-density lipoprotein-induced microparticles promote endothelial monocyte adhesion via intercellular adhesion molecule 1.

Fu Z, Zhou E, Wang X, Tian M, Kong J, Li J, Ji L, Niu C, Shen H, Dong S, Liu C, Vermorken A, Willard B, Zu L, Zheng L
American journal of physiology. Cell physiology 2017 Nov 1;313(5):C567-C574

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs.

Hu G, Wang J, Hong D, Zhang T, Duan H, Mu X, Yang Z
BMC complementary and alternative medicine 2017 Jan 11;17(1):38

Perivascular adipose tissue alleviates inflammatory factors and stenosis in diabetic blood vessels.

Li T, Liu X, Ni L, Wang Z, Wang W, Shi T, Liu X, Liu C
Biochemical and biophysical research communications 2016 Nov 11;480(2):147-152

The protective effect of melatonin on smoke-induced vascular injury in rats and humans: a randomized controlled trial.

Wang Z, Ni L, Wang J, Lu C, Ren M, Han W, Liu C
Journal of pineal research 2016 Mar;60(2):217-27

Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb.

Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X
International journal of clinical and experimental pathology 2015;8(4):3451-9

Comparison of protective effects of safflor injection and extract of Ginkgo biloba on lung ischemia/reperfusion injury in rabbits.

Tian XX, Wang BL, Cao YZ, Zhong YX, Tu YY, Xiao JB, He QF, Zhai LN
Chinese journal of integrative medicine 2015 Mar;21(3):229-33

Retinoic Acid promotes interleukin-4 plasmid-dimethylsulfoxide topical transdermal delivery for treatment of psoriasis.

Chen ZW, Zhang YB, Chen XJ, Liu X, Wang Z, Zhou XK, Qiu J, Zhang NN, Teng X, Mao YQ, Liu CY, Wei YQ, Li J
Annals of dermatology 2015 Apr;27(2):121-7

Accumulation of FLT3(+) CD11c (+) dendritic cells in psoriatic lesions and the anti-psoriatic effect of a selective FLT3 inhibitor.

Yan HX, Li WW, Zhang Y, Wei XW, Fu LX, Shen GB, Yin T, Li XY, Shi HS, Wan Y, Zhang QY, Li J, Yang SY, Wei YQ
Immunologic research 2014 Oct;60(1):112-26

Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury.

Yang GH, Li YC, Wang ZQ, Liu B, Ye W, Ni L, Zeng R, Miao SY, Wang LF, Liu CW
Journal of pineal research 2014 Nov;57(4):451-8

Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.

Huang KF, Huang XP, Xiao GQ, Yang HY, Lin JS, Diao Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 May;68(4):455-61

Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats.

Lin Y, Liu HL, Fang J, Yu CH, Xiong YK, Yuan K
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Jun;68:290-6

Glycyrrhizin protects against porcine endotoxemia through modulation of systemic inflammatory response.

Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H
Critical care (London, England) 2013 Mar 11;17(2):R44

Calycosin protects HUVECs from advanced glycation end products-induced macrophage infiltration.

Xu Y, Feng L, Wang S, Zhu Q, Zheng Z, Xiang P, He B, Tang D
Journal of ethnopharmacology 2011 Sep 1;137(1):359-70

Curcumin protects mice against concanavalin A-induced hepatitis by inhibiting intrahepatic intercellular adhesion molecule-1 (ICAM-1) and CXCL10 expression.

Tu CT, Han B, Liu HC, Zhang SC
Molecular and cellular biochemistry 2011 Dec;358(1-2):53-60

Protective effect and mechanism of sodium tanshinone II A sulfonate on microcirculatory disturbance of small intestine in rats with sepsis.

Zhu W, Lv Q, Chen H, Wang Z, Zhong Q
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2011 Aug;31(4):441

Protective effect of carnosol on lung injury induced by intestinal ischemia/reperfusion.

Tian XF, Yao JH, Zhang XS, Zheng SS, Guo XH, Wang LM, Wang ZZ, Liu KX
Surgery today 2010 Sep;40(9):858-65

Gene therapy for psoriasis in the K14-VEGF transgenic mouse model by topical transdermal delivery of interleukin-4 using ultradeformable cationic liposome.

Li J, Li X, Zhang Y, Zhou XK, Yang HS, Chen XC, Wang YS, Wei YQ, Chen LJ, Hu HZ, Liu CY
The journal of gene medicine 2010 Jun;12(6):481-90

Blockade of PKC-beta protects HUVEC from advanced glycation end products induced inflammation.

Xu Y, Wang S, Feng L, Zhu Q, Xiang P, He B
International immunopharmacology 2010 Dec;10(12):1552-9

A novel conjugate of low-molecular-weight heparin and Cu,Zn-superoxide dismutase: study on its mechanism in preventing brain reperfusion injury after ischemia in gerbils.

Qi J, Li Y, Zhang H, Cheng Y, Sung Y, Cao J, Zhao Y, Wang F
Brain research 2009 Mar 13;1260:76-83

Protective effect of JBP485 on concanavalin A-induced liver injury in mice.

Yang T, Wu J, Wang C, Liu Q, Ma X, Peng J, Kaku T, Liu K
The Journal of pharmacy and pharmacology 2009 Jun;61(6):767-74

Laennec protects murine from concanavalin A-induced liver injury through inhibition of inflammatory reactions and hepatocyte apoptosis.

Wu J, Yang T, Wang C, Liu Q, Yao J, Sun H, Kaku T, Liu KX
Biological & pharmaceutical bulletin 2008 Nov;31(11):2040-4

Protective effect of bicyclol on acute hepatic failure induced by lipopolysaccharide and D-galactosamine in mice.

Wang H, Li Y
European journal of pharmacology 2006 Mar 18;534(1-3):194-201

Effect of systemic LPS injection on cortical NF-kappaB activity and inflammatory response following traumatic brain injury in rats.

Hang CH, Shi JX, Tian J, Li JS, Wu W, Yin HX
Brain research 2004 Nov 5;1026(1):23-32

Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats.

Deng S, Deng PY, Jiang JL, Ye F, Yu J, Yang TL, Deng HD, Li YJ
Acta pharmacologica Sinica 2004 Dec;25(12):1633-9

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Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,Lane 2: rat thymus tissue lysate,Lane 3: rat RH35 cell lysate,Lane 4: mouse spleen tissue lysate,Lane 5: mouse thymus tissue lysate,Lane 6: mouse HEPA1-6 cell lysate,Lane 7: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
Additional image