Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-88700 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88700, RRID:AB_11029978
- Product name
- Rabbit Polyclonal ICAM-1/CD54 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ICAM-1/CD54 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in control (vector only transfected HEK293T lysate) and ICAM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88700 (A) shows similar protein distribution across tissues to independent antibody NBP1-88701 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lymph node shows moderate membranous positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lung shows moderate membranous positivity in pneumocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.