MA1-19123

antibody from Invitrogen Antibodies

Targeting: ICAM1 BB2, CD54

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19123

Provider

Invitrogen Antibodies

Product name

ICAM-1 Monoclonal Antibody (MEM-111)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. Western Blot: non-reducing conditions. Immunohistochemistry (paraffin sections): positive tissue: thymus, RE cells.

Reactivity

Human, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

MEM-111

Vial size

100 μg

Concentration

1 mg/mL

Storage

4°C, do not freeze

References

Submitted references

Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.

Basehore SE, Bohlman S, Weber C, Swaminathan S, Zhang Y, Jang C, Arany Z, Clyne AM
Circulation research 2021 Nov 12;129(11):1054-1066

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Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation.

Taechangam N, Iyer SS, Walker NJ, Arzi B, Borjesson DL
Stem cell research & therapy 2019 Jun 25;10(1):188

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Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting.

Fromm JR, Thomas A, Wood BL
The American journal of pathology 2017 Feb;187(2):304-317

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Integrated pathways for neutrophil recruitment and inflammation in leprosy.

Lee DJ, Li H, Ochoa MT, Tanaka M, Carbone RJ, Damoiseaux R, Burdick A, Sarno EN, Rea TH, Modlin RL
The Journal of infectious diseases 2010 Feb 15;201(4):558-69

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LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.

Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8128-36

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LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.

Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8128-36

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Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.

Fromm JR, Kussick SJ, Wood BL
American journal of clinical pathology 2006 Nov;126(5):764-80

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Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.

Zhang F, Marcus WD, Goyal NH, Selvaraj P, Springer TA, Zhu C
The Journal of biological chemistry 2005 Dec 23;280(51):42207-18

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Filarial antigens impair the function of human dendritic cells during differentiation.

Semnani RT, Sabzevari H, Iyer R, Nutman TB
Infection and immunity 2001 Sep;69(9):5813-22

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Filarial antigens impair the function of human dendritic cells during differentiation.

Semnani RT, Sabzevari H, Iyer R, Nutman TB
Infection and immunity 2001 Sep;69(9):5813-22

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ICAM-1 using ICAM-1 Monoclonal Antibody (MEM-111) (Product # MA1-19123). Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 μg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ICAM-1 using ICAM-1 Monoclonal Antibody (MEM-111) (Product # MA1-19123). Flow cytometry surface staining pattern of human peripheral blood stained using anti-human ICAM-1 (MEM-111) purified antibody (concentration in sample 0.6 μg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ICAM-1 using ICAM-1 Monoclonal Antibody (MEM-111) (Product # MA1-19123). Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 μg/mL, GAM APC).