Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ICAM-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Rhesus Monkey (85%), Chimpanzee (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ICAM-1 Polyclonal Antibody (Product # PA5-27189). Sample (30 µg of whole cell lysate). Lane A: HeLa. 7.5% SDS PAGE. ICAM-1 Polyclonal Antibody (Product # PA5-27189) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ICAM1/CD54 antibody [N1C2] detects ICAM1/CD54 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: ICAM1/CD54 stained by ICAM1/CD54 antibody [N1C2] (Product # PA5-27189) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1/CD54 (Product # PA5-27189) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.