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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF720 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ICAM-1/CD54 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Selected for its ability to block the adhesion of HSB-2 cells to immobilized recombinant human (rh) ICAM-1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM-1, recombinant mouse ICAM-1, and rhVCAM-1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction.
High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.
Genshaft AS, Li S, Gallant CJ, Darmanis S, Prakadan SM, Ziegler CG, Lundberg M, Fredriksson S, Hong J, Regev A, Livak KJ, Landegren U, Shalek AK
Genome biology 2016 Sep 19;17(1):188
Genome biology 2016 Sep 19;17(1):188
High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
Hashizume M, Mihara M
Biochemical and biophysical research communications 2010 Dec 10;403(2):184-9
Biochemical and biophysical research communications 2010 Dec 10;403(2):184-9
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
Journal of leukocyte biology 2008 Oct;84(4):1109-19
Journal of leukocyte biology 2008 Oct;84(4):1109-19
Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
Schellerer VS, Croner RS, Weinländer K, Hohenberger W, Stürzl M, Naschberger E
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1159-70
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1159-70
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis and rheumatism 2007 Jun;56(6):1817-26
Arthritis and rheumatism 2007 Jun;56(6):1817-26
Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.
Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R
Blood 2007 Feb 15;109(4):1381-6
Blood 2007 Feb 15;109(4):1381-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human ICAM-1/CD54 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA) for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ICAM-1/CD54 in A431 Human Cell Line. ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line (left panel; positive stain) and A549 human lung carcinoma cell line (right panel; negative stain) using Sheep Anti-Human ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
