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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
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- Product number
- BBA20 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ICAM-1/CD54 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human ICAM-1/CD54 on human ICAM-1 transfected COS cells. It does not bind COS cells transfected with E-Selectin, VCAM-1, or PECAM-1.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BBIG-I1 (11C81)
- Vial size
- 100 Tests
- Concentration
- LYOPH
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps.
SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease.
Tranexamic acid and the gut barrier: Protection by inhibition of trypsin uptake and activation of downstream intestinal proteases.
Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro.
Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells.
The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles.
Molecular mechanisms regulating the synergism between IL-32γ and NOD for the activation of eosinophils.
Transendothelial migration enables subsequent transmigration of neutrophils through underlying pericytes.
TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
Tengroth L, Arebro J, Larsson O, Bachert C, Georén SK, Cardell LO
Scientific reports 2018 Jan 24;8(1):1561
Scientific reports 2018 Jan 24;8(1):1561
SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease.
Chan SH, Hung CH, Shih JY, Chu PM, Cheng YH, Lin HC, Tsai KL
Redox biology 2017 Oct;13:301-309
Redox biology 2017 Oct;13:301-309
Tranexamic acid and the gut barrier: Protection by inhibition of trypsin uptake and activation of downstream intestinal proteases.
Diebel ME, Diebel LN, Liberati DM
American journal of surgery 2017 Mar;213(3):489-493
American journal of surgery 2017 Mar;213(3):489-493
Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro.
Schaefer A, van Duijn TJ, Majolee J, Burridge K, Hordijk PL
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4823-4836
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4823-4836
Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells.
Rouger C, Pagie S, Derbré S, Le Ray AM, Richomme P, Charreau B
PloS one 2016;11(12):e0167361
PloS one 2016;11(12):e0167361
The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles.
Happonen KE, Tran S, Mörgelin M, Prince R, Calzavarini S, Angelillo-Scherrer A, Dahlbäck B
The Journal of biological chemistry 2016 May 13;291(20):10586-601
The Journal of biological chemistry 2016 May 13;291(20):10586-601
Molecular mechanisms regulating the synergism between IL-32γ and NOD for the activation of eosinophils.
Wong CK, Dong J, Lam CW
Journal of leukocyte biology 2014 Apr;95(4):631-42
Journal of leukocyte biology 2014 Apr;95(4):631-42
Transendothelial migration enables subsequent transmigration of neutrophils through underlying pericytes.
Ayres-Sander CE, Lauridsen H, Maier CL, Sava P, Pober JS, Gonzalez AL
PloS one 2013;8(3):e60025
PloS one 2013;8(3):e60025
TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
Stephan D, Sbai O, Wen J, Couraud PO, Putterman C, Khrestchatisky M, Desplat-Jégo S
Journal of neuroinflammation 2013 Jan 15;10:9
Journal of neuroinflammation 2013 Jan 15;10:9
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
Pennino D, Bhavsar PK, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham SR, Cavani A, Eyerich K, Chung KF, Schmidt-Weber CB, Eyerich S
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
Liu KY, Hu S, Chan BC, Wat EC, Lau CB, Hon KL, Fung KP, Leung PC, Hui PC, Lam CW, Wong CK
Molecules (Basel, Switzerland) 2013 Feb 25;18(3):2483-500
Molecules (Basel, Switzerland) 2013 Feb 25;18(3):2483-500
Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
Li N, Mao D, Lü S, Tong C, Zhang Y, Long M
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):4371-81
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 15;190(8):4371-81
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Wilhelmsen K, Mesa KR, Lucero J, Xu F, Hellman J
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
PloS one 2010 Oct 21;5(10):e13568
PloS one 2010 Oct 21;5(10):e13568
IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
Marino M, Scuderi F, Provenzano C, Scheller J, Rose-John S, Bartoccioni E
Journal of neuroimmunology 2008 May 30;196(1-2):41-8
Journal of neuroimmunology 2008 May 30;196(1-2):41-8
Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
Gutiérrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montaño LF, López-Marure R
Atherosclerosis 2007 Jan;190(1):90-9
Atherosclerosis 2007 Jan;190(1):90-9
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
Journal of leukocyte biology 2003 Apr;73(4):525-9
Journal of leukocyte biology 2003 Apr;73(4):525-9
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
Journal of leukocyte biology 2003 Apr;73(4):525-9
Journal of leukocyte biology 2003 Apr;73(4):525-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ICAM-1/CD54 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG1 Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
