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- Antibody Data
- Antigen structure
- References [42]
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- Immunocytochemistry [1]
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- Product number
- BBA3 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ICAM-1/CD54 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- BBIG-I1 (11C81)
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dual complementary liposomes inhibit triple-negative breast tumor progression and metastasis.
Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis.
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Pinocembrin protects endothelial cells from oxidized LDL-induced injury.
Inflammation-Sensitive Myosin-X Functionally Supports Leukocyte Extravasation by Cdc42-Mediated ICAM-1-Rich Endothelial Filopodia Formation.
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts.
A quantitative method for screening and identifying molecular targets for nanomedicine.
A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification.
Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro.
Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells.
Role of cortactin homolog HS1 in transendothelial migration of natural killer cells.
C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCζ tumor-suppressive activities and regulating integrin affinity modulation.
Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
Uveal melanoma cells utilize a novel route for transendothelial migration.
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD.
Tumor-associated fibroblasts isolated from colorectal cancer tissues exhibit increased ICAM-1 expression and affinity for monocytes.
Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-κB pathway.
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction.
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis.
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
Differential effects of orbital and laminar shear stress on endothelial cells.
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Apical topography and modulation of ICAM-1 expression on activated endothelium.
Guo P, Yang J, Liu D, Huang L, Fell G, Huang J, Moses MA, Auguste DT
Science advances 2019 Mar;5(3):eaav5010
Science advances 2019 Mar;5(3):eaav5010
Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis.
Blatchley MR, Hall F, Wang S, Pruitt HC, Gerecht S
Science advances 2019 Mar;5(3):eaau7518
Science advances 2019 Mar;5(3):eaau7518
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, Adams Y
PloS one 2018;13(10):e0204177
PloS one 2018;13(10):e0204177
Pinocembrin protects endothelial cells from oxidized LDL-induced injury.
Su Q, Sun Y, Ye Z, Yang H, Kong B, Li L
Cytokine 2018 Nov;111:475-480
Cytokine 2018 Nov;111:475-480
Inflammation-Sensitive Myosin-X Functionally Supports Leukocyte Extravasation by Cdc42-Mediated ICAM-1-Rich Endothelial Filopodia Formation.
Kroon J, Schaefer A, van Rijssel J, Hoogenboezem M, van Alphen F, Hordijk P, Stroes ESG, Strömblad S, van Rheenen J, van Buul JD
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1790-1801
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1790-1801
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994
Molecular medicine reports 2018 Aug;18(2):1987-1994
Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts.
Raschi E, Chighizola CB, Cesana L, Privitera D, Ingegnoli F, Mastaglio C, Meroni PL, Borghi MO
Arthritis research & therapy 2018 Aug 29;20(1):187
Arthritis research & therapy 2018 Aug 29;20(1):187
A quantitative method for screening and identifying molecular targets for nanomedicine.
Guo P, Yang J, Bielenberg DR, Dillon D, Zurakowski D, Moses MA, Auguste DT
Journal of controlled release : official journal of the Controlled Release Society 2017 Oct 10;263:57-67
Journal of controlled release : official journal of the Controlled Release Society 2017 Oct 10;263:57-67
A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification.
Arebro J, Ekstedt S, Hjalmarsson E, Winqvist O, Kumlien Georén S, Cardell LO
Scientific reports 2017 Mar 8;7:43568
Scientific reports 2017 Mar 8;7:43568
Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro.
Schaefer A, van Duijn TJ, Majolee J, Burridge K, Hordijk PL
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4823-4836
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4823-4836
Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells.
Ganesan MK, Finsterwalder R, Leb H, Resch U, Neumüller K, de Martin R, Petzelbauer P
Tissue engineering. Part C, Methods 2017 Jan;23(1):38-49
Tissue engineering. Part C, Methods 2017 Jan;23(1):38-49
Role of cortactin homolog HS1 in transendothelial migration of natural killer cells.
Mukherjee S, Kim J, Mooren OL, Shahan ST, Cohan M, Cooper JA
PloS one 2015;10(2):e0118153
PloS one 2015;10(2):e0118153
C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCζ tumor-suppressive activities and regulating integrin affinity modulation.
Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
Scientific reports 2015 Mar 20;5:9275
Scientific reports 2015 Mar 20;5:9275
Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
Malara NM, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V
International journal of cardiology. Heart & vasculature 2015 Mar 1;6:48-53
International journal of cardiology. Heart & vasculature 2015 Mar 1;6:48-53
West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
Roe K, Orillo B, Verma S
PloS one 2014;9(7):e102598
PloS one 2014;9(7):e102598
Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
PloS one 2014;9(4):e94860
PloS one 2014;9(4):e94860
Uveal melanoma cells utilize a novel route for transendothelial migration.
Onken MD, Li J, Cooper JA
PloS one 2014;9(12):e115472
PloS one 2014;9(12):e115472
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Lauridsen HM, Pober JS, Gonzalez AL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1166-80
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1166-80
Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD.
Noviello M, Tedesco FS, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli MFM, Marktel S, Napolitano S, Cicalese MP, Ciceri F, Peretti G, Cossu G, Bonini C
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1342-1352
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1342-1352
Tumor-associated fibroblasts isolated from colorectal cancer tissues exhibit increased ICAM-1 expression and affinity for monocytes.
Schellerer VS, Langheinrich M, Hohenberger W, Croner RS, Merkel S, Rau TT, Stürzl M, Naschberger E
Oncology reports 2014 Jan;31(1):255-61
Oncology reports 2014 Jan;31(1):255-61
Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
Mooren OL, Li J, Nawas J, Cooper JA
Molecular biology of the cell 2014 Dec 15;25(25):4115-29
Molecular biology of the cell 2014 Dec 15;25(25):4115-29
Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
Ohnuma K, Fujiki A, Yanagihara K, Tachikawa S, Hayashi Y, Ito Y, Onuma Y, Chan T, Michiue T, Furue MK, Asashima M
Scientific reports 2014 Apr 11;4:4646
Scientific reports 2014 Apr 11;4:4646
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
Park SJ, Kim KJ, Kim WU, Cho CS
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
Lin YW, Huang CY, Chen YH, Shih CM, Tsao NW, Lin CY, Chang NC, Tsai CS, Tsai HY, Tsai JC, Huang PH, Li CY, Lin FY
PloS one 2013;8(12):e84731
PloS one 2013;8(12):e84731
Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-κB pathway.
Tsai SH, Huang PH, Peng YJ, Chang WC, Tsai HY, Leu HB, Chen JW, Lin SJ
Cardiovascular research 2013 Dec 1;100(3):501-10
Cardiovascular research 2013 Dec 1;100(3):501-10
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Xu W, Joo H, Clayton S, Dullaers M, Herve MC, Blankenship D, De La Morena MT, Balderas R, Picard C, Casanova JL, Pascual V, Oh S, Banchereau J
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Wilhelmsen K, Mesa KR, Lucero J, Xu F, Hellman J
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
The Journal of biological chemistry 2012 Aug 3;287(32):26478-94
Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction.
Stroka KM, Aranda-Espinoza H
Blood 2011 Aug 11;118(6):1632-40
Blood 2011 Aug 11;118(6):1632-40
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
Journal of hepatology 2009 Oct;51(4):734-49
Journal of hepatology 2009 Oct;51(4):734-49
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
Journal of leukocyte biology 2007 Mar;81(3):748-56
Journal of leukocyte biology 2007 Mar;81(3):748-56
Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis.
Bae E, Kim WJ, Kang YM, Suk K, Koh EM, Cha HS, Ahn KS, Huh TL, Lee WH
Clinical and experimental immunology 2007 Jun;148(3):410-8
Clinical and experimental immunology 2007 Jun;148(3):410-8
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
Rajesh M, Mukhopadhyay P, Bátkai S, Haskó G, Liaudet L, Drel VR, Obrosova IG, Pacher P
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H610-9
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H610-9
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
Blood 2006 Mar 15;107(6):2252-61
Blood 2006 Mar 15;107(6):2252-61
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
Ramachandran R, Morice AH, Compton SJ
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
Tamai R, Asai Y, Ogawa T
Infection and immunity 2005 Oct;73(10):6290-8
Infection and immunity 2005 Oct;73(10):6290-8
Differential effects of orbital and laminar shear stress on endothelial cells.
Dardik A, Chen L, Frattini J, Asada H, Aziz F, Kudo FA, Sumpio BE
Journal of vascular surgery 2005 May;41(5):869-80
Journal of vascular surgery 2005 May;41(5):869-80
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Yamamoto H, Nagata M, Sakamoto Y
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2005 Feb;94(2):292-300
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2005 Feb;94(2):292-300
Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
Domeij H, Modéer T, Quezada HC, Yucel-Lindberg T
Biochemical and biophysical research communications 2005 Dec 30;338(4):1825-33
Biochemical and biophysical research communications 2005 Dec 30;338(4):1825-33
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Helanterä I, Loginov R, Koskinen P, Törnroth T, Grönhagen-Riska C, Lautenschlager I
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2005 Apr;20(4):790-6
Apical topography and modulation of ICAM-1 expression on activated endothelium.
Almenar-Queralt A, Duperray A, Miles LA, Felez J, Altieri DC
The American journal of pathology 1995 Nov;147(5):1278-88
The American journal of pathology 1995 Nov;147(5):1278-88
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- ICAM-1/CD54 in HUVEC Cells. ICAM-1/CD54 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
