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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [4]
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- Product number
- PA5-27909 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Vitronectin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 conditioned medium, mouse brain.
- Concentration
- 0.34 mg/mL
Submitted references Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin α (v) in Glioblastoma Cells.
Candidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays.
Yu Q, Xiao W, Sun S, Sohrabi A, Liang J, Seidlits SK
Frontiers in cell and developmental biology 2021;9:616580
Frontiers in cell and developmental biology 2021;9:616580
Candidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays.
Oungsakul P, Choi E, Shah AK, Mohamed A, O'Leary C, Duffy D, Hill MM, Bielefeldt-Ohmann H
Veterinary sciences 2021 Feb 27;8(3)
Veterinary sciences 2021 Feb 27;8(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Vitronectin using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Vitronectin polyclonal antibody (Product # PA5-27909) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Vitronectin was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Vitronectin Polyclonal Antibody (Product # PA5-27909) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Vitronectin Polyclonal Antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin Polyclonal Antibody (Product # PA5-27909) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vitronectin (Product # PA5-27909) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Vitronectin Polyclonal Antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin Polyclonal Antibody (Product # PA5-27909) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Lectin-histochemistry and immunohistochemical labelling of canine splenic HSA. Formalin-fixed paraffin-embedded (FFPE) sections were reacted with anti-human CD31 antibody, anti-human VTN antibody, anti-human complement C7 antibody, and biotinylated lectins DSA ( Datura stramonium ), WGA (Wheat Germ Agglutinin), and SNA ( Sambucus nigra ) lectins) on consecutive splenic HSA sections (sample 13_019400E). Binding was visualized using AEC substrate (red) following either incubation with secondary antibody (Envision Kit) or streptavidin-horse radish peroxidase conjugate. Microphotos were generated using Aperio scans. Original magnifications 20x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Boxplot representing overall lectin-histochemistry (DSA, WGA, SNA) and immunohistochemical (CD31, VTN, C7) signal intensities. Three tissue components-HSA/cancer, arterial endothelium, and venous endothelium-were assessed in two sample groups-dogs with HSA and dogs without HSA. Differences in the level of tissue glycoprotein expression between tissue components and between HSA and non-HSA were compared.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Boxplots demonstrate the comparison of H-scores assessed from lectin-histochemistry (DSA, WGA, SNA) and immunohistochemical (CD31, VTN, C7) labelling of tissue samples. The statistical analysis was performed by comparing H-scores between tissue components-HSA/cancer cells, arterial endothelium (AE) and venous endothelium (VE). Significance bars show only p -values < 0.05 derived from Wilcoxon test and adjusted by Bonferroni corrections.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Scatter plots of H-scores distribution, assessed from lectin-immunohistochemical labelling for complement C7 and VTN as well as DSA, WGA, and SNA. ( A ) Matrix scatter plots created for the candidate antibodies/lectins H-scores. The darker dots (dark brown) represent H-score assessed from the cancer component of HSA splenic tissue samples, while the lighter dots (light green) represent H-score assessed from the HSA-like lesion of non-HSA (hematoma and hemorrhage) splenic tissue samples. ( B ) Scatter plot of H-scores distribution assessed from lectin-immunohistochemical labelling with the anti-human complement C7 antibody and DSA lectin. The pair of reactants was selected from recursive partitioning results. Two cut-off points, complement C7 (H-score > 67.5) then DSA (H-score > 145), allow clear distinction between HSA and HSA-like tissues.
