PA5-27909

antibody from Invitrogen Antibodies

Targeting: VTN VN

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-27909 - Provider product page
Provider: Invitrogen Antibodies
Product name: Vitronectin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2 conditioned medium, mouse brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.34 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

VTN protein structure - PA5-27909 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Lectin-histochemistry and immunohistochemical labelling of canine splenic HSA. Formalin-fixed paraffin-embedded (FFPE) sections were reacted with anti-human CD31 antibody, anti-human VTN antibody, anti-human complement C7 antibody, and biotinylated lectins DSA ( Datura stramonium ), WGA (Wheat Germ Agglutinin), and SNA ( Sambucus nigra ) lectins) on consecutive splenic HSA sections (sample 13_019400E). Binding was visualized using AEC substrate (red) following either incubation with secondary antibody (Envision Kit) or streptavidin-horse radish peroxidase conjugate. Microphotos were generated using Aperio scans. Original magnifications 20x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Boxplot representing overall lectin-histochemistry (DSA, WGA, SNA) and immunohistochemical (CD31, VTN, C7) signal intensities. Three tissue components-HSA/cancer, arterial endothelium, and venous endothelium-were assessed in two sample groups-dogs with HSA and dogs without HSA. Differences in the level of tissue glycoprotein expression between tissue components and between HSA and non-HSA were compared.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Boxplots demonstrate the comparison of H-scores assessed from lectin-histochemistry (DSA, WGA, SNA) and immunohistochemical (CD31, VTN, C7) labelling of tissue samples. The statistical analysis was performed by comparing H-scores between tissue components-HSA/cancer cells, arterial endothelium (AE) and venous endothelium (VE). Significance bars show only p -values < 0.05 derived from Wilcoxon test and adjusted by Bonferroni corrections.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Scatter plots of H-scores distribution, assessed from lectin-immunohistochemical labelling for complement C7 and VTN as well as DSA, WGA, and SNA. ( A ) Matrix scatter plots created for the candidate antibodies/lectins H-scores. The darker dots (dark brown) represent H-score assessed from the cancer component of HSA splenic tissue samples, while the lighter dots (light green) represent H-score assessed from the HSA-like lesion of non-HSA (hematoma and hemorrhage) splenic tissue samples. ( B ) Scatter plot of H-scores distribution assessed from lectin-immunohistochemical labelling with the anti-human complement C7 antibody and DSA lectin. The pair of reactants was selected from recursive partitioning results. Two cut-off points, complement C7 (H-score > 67.5) then DSA (H-score > 145), allow clear distinction between HSA and HSA-like tissues.