MA1-34500

antibody from Invitrogen Antibodies

Targeting: ICAM2 CD102

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-34500

Provider

Invitrogen Antibodies

Product name

ICAM-2 Monoclonal Antibody (B-T1)

Antibody type

Monoclonal

Antigen

Other

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B-T1

Vial size

200 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ICAM-2 was performed using log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ICAM-2 Mouse Monoclonal Antibody (B-T1) (Product # MA1-34500) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.