Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF774 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ICAM-2/CD102 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse ICAM-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ICAM-2 and recombinant rat ICAM-1 and less than 1% cross-reactivity with recombinant mouse (rm) VCAM-1, rmICAM-5, and rmICAM-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice.
Porras G, Ayuso MS, González-Manchón C
The international journal of biochemistry & cell biology 2018 Jun;99:72-79
The international journal of biochemistry & cell biology 2018 Jun;99:72-79
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse ICAM-2/CD102 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ICAM-2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for ICAM-2/CD102 at approximately 50 to 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ICAM-2/CD102 in Mouse Ovary. ICAM-2/CD102 was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse ICAM-2/CD102 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF774) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
