Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-1029-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD102 (ICAM-2) Monoclonal Antibody (CBR-IC2/2), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity. Applications Reported: This CBRIC2/2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CBRIC2/2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- CBR-IC2/2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.
Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation.
Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
Diacovo TG, deFougerolles AR, Bainton DF, Springer TA
The Journal of clinical investigation 1994 Sep;94(3):1243-51
The Journal of clinical investigation 1994 Sep;94(3):1243-51
Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation.
Butini L, De Fougerolles AR, Vaccarezza M, Graziosi C, Cohen DI, Montroni M, Springer TA, Pantaleo G, Fauci AS
European journal of immunology 1994 Sep;24(9):2191-5
European journal of immunology 1994 Sep;24(9):2191-5
Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
de Fougerolles AR, Stacker SA, Schwarting R, Springer TA
The Journal of experimental medicine 1991 Jul 1;174(1):253-67
The Journal of experimental medicine 1991 Jul 1;174(1):253-67
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (open histogram) or Anti-Human CD102 (ICAM-2) FITC (filled histogram). Cells in the lymphocyte (left) or monocyte (right) gates were used for analysis.
- Conjugate
- Green dye
