MA1-26736

antibody from Invitrogen Antibodies

Targeting: HNRNPA1 ALS20, hnRNP-A1, HNRPA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-26736

Provider

Invitrogen Antibodies

Product name

hnRNP A1 Monoclonal Antibody (9H10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

Recommended positive controls: 293T. A band of approximately 34 kDa is detected by Western Blot.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster

Host

Mouse

Isotype

IgG

Antibody clone number

9H10

Vial size

50 µL

Concentration

2.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Comparative analyses of alphaviral RNA:Protein complexes reveals conserved host-pathogen interactions.

Gebhart NN, Hardy RW, Sokoloski KJ
PloS one 2020;15(8):e0238254

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MicroRNA-132 loss is associated with tau exon 10 inclusion in progressive supranuclear palsy.

Smith PY, Delay C, Girard J, Papon MA, Planel E, Sergeant N, Buée L, Hébert SS
Human molecular genetics 2011 Oct 15;20(20):4016-24

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In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs.

Smith P, Al Hashimi A, Girard J, Delay C, Hébert SS
Journal of neurochemistry 2011 Jan;116(2):240-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hnRNP-A1 in (lane 1) HEK-293T (lane 2) HeLa (lane 3) G361 (lane 4) COS7 (lane 5) MDBK (lane 6) NRK (lane 7) CHO lysate. Samples were probed with a hnRNP-A1 monoclonal antibody (Product # MA1-26736) at a dilution of 2µg/mL and developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hnRNP-A1 using a hnRNP-A1 monoclonal antibody (Product # MA1-26736).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of hnRNP A1 was performed by loading (1)HEK-293T (2) HeLa (3) G361 (4) COS7 (5) MDBK (6) NRK (7) CHO cells. Proteins were transferred to a membrane and probed with a hnRNP A1 Monoclonal Antibody (9H10) (Product # MA1-26736) at a dilution of 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of hnRNP A1 was performed by loading (1)HEK-293T (2) HeLa (3) G361 (4) COS7 (5) MDBK (6) NRK (7) CHO cells. Proteins were transferred to a membrane and probed with a hnRNP A1 Monoclonal Antibody (9H10) (Product # MA1-26736) at a dilution of 1 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of hnRNP A1 in HeLa cells using hnRNP A1 Monoclonal Antibody (9H10) (Product # MA1 26736) at 10 µg/mL. Cells were fixed and permeabilized with cold methanol followed by cold methanol: acetone.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of hnRNP A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with hnRNP A1 Monoclonal Antibody (9H10) (Product # MA1-26736) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RNA immunoprecipitation (RIP) western of hnRNP A1 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of monoclonal hnRNP A1 antibody (Product # MA1-26736) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-mouse coated Dynabeads (Product # 11202D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RNA immunoprecipitation (RIP) western of hnRNP A1 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of monoclonal hnRNP A1 antibody (Product # MA1-26736) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-mouse coated Dynabeads (Product # 11202D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.