11176-1-AP

antibody from Proteintech Group

Targeting: HNRNPA1 ALS20, hnRNP-A1, HNRPA1

Provider product page for 11176-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 11176-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11176-1-AP, RRID:AB_2117177
Product name: HNRNPA1 antibody
Antibody type: Polyclonal
Description: KD/KO validated HNRNPA1 antibody (Cat. #11176-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, pig and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Porcine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HNRNPA1 protein structure - 11176-1-AP shown in red.

Submitted references Blocking ubiquitination of hnRNPA1 maintains the self-renewal of breast cancer stem cells via mutually exclusive splicing of PKM pre-mRNA.

Lv X, Han L, Tong WW, Sun X, Xu S, Yan Y, Zhou S, Zhang Y, Zhang D, Wang J, Liu J, Zhao H, Yao W, Xiao R, Wang Q, Xu J, Gong L, Wei M, Chen B, He M
Oncogene 2026 Jun;45(20):1914-1929

Multiplex neurodegeneration proteotoxicity platform reveals DNAJB6 promotes non-toxic FUS condensate gelation and inhibits neurotoxicity.

Resnick SJ, Qamar S, Krishna P, Korobeynikov V, Ausserwoger H, Miller A, Esposito P, Varela JA, Sheng J, Huang LH, Nixon-Abell J, Melore S, Chung CW, Läubli NF, Kapsiani S, Li X, Wang J, Zhang N, Alam MM, Burguete AS, Swayne TC, Chen Y, Liao YC, Shneider NA, Vendruscolo M, Knowles TPJ, Kaminski CF, Ruggeri FS, Kaminski Schierle GS, St George-Hyslop P, Chavez A
Nature communications 2025 Nov 21;16(1):10285

Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA.

Chen Z, Zhou Y, Xue C, Zeng L, Deng S, Xu Z, Li M, Zhao H, He X, Liu S, Liu J, Liu S, Zhao S, Zhang S, Peng X, Wu X, Bai R, Zhuang L, Wu S, Zhang J, Lin D, Huang X, Zheng J
Nature cell biology 2025 Jun;27(6):1035-1047

A novel polypeptide encoded by circSPIRE1 promotes prostate cancer proliferation and migration by restraining the ubiquitin-dependent degradation of LRP5.

Hu J, Shi J, Wang J, Xiao Y, Kong D, Gao M, Luo T, Xu S, Yuan Z, Ma X, Dong X, Huang J, Liu C, Xu K
Journal of experimental & clinical cancer research : CR 2025 Jul 25;44(1):218

Altered mRNA transport and local translation in i3Neurons with RNA-binding protein knockdown.

Dargan R, Mikheenko A, Johnson NL, Packer BE, Li Z, Craig EJ, Como CN, Sarbanes SL, Bereda C, Hao Y, Mehta PR, Keuss M, Nalls MA, Qi YA, Weller CA, Fratta P, Ryan VH
Nucleic acids research 2025 Jul 19;53(14)

CircSMAD3 represses VSMC phenotype switching and neointima formation via promoting hnRNPA1 ubiquitination degradation.

Mei S, Ma X, Zhou L, Wuyun Q, Cai Z, Yan J, Ding H
Cell proliferation 2025 Jan;58(1):e13742

Abnormal ac4C modification in metabolic dysfunction associated steatotic liver cells.

Zhang X, Zheng Y, Yang J, Yang Y, He Q, Xu M, Long F, Yang Y
Scientific reports 2025 Jan 6;15(1):1013

The UP1 domain is essential for the facilitation effect of HnRNP A1 on PRRSV-2 replication.

Jing H, Liu Y, Peng Z, Duan E, Liu J, Lv Y, Sun Y, Dong W, Li X, Wang J, Cao S, Wang H, Zhang Y, Li H
Virology 2025 Feb;603:110378

DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation.

Gupta A, Bansal M, Ding J, Karki S, Pandit M, Sudarshan S, Ding HF
Science advances 2025 Aug 29;11(35):eadv9401

Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression.

Hu Y, Li Y, Xiong H, Zhang Y, Wang F, Zhuo W, Zeng Z, Zhao Y, Wang H, Hu P, Han S, Huang Y, Lv G, Zhao G
International journal of biological sciences 2024;20(11):4341-4363

Inhibition of heterogeneous nuclear ribonucleoproteins A1 and oxidative stress reduces glycolysis via pyruvate kinase M2 in chronic thromboembolic pulmonary hypertension.

Liu L, Pang W, Liu J, Xu S, Zhang Z, Hao R, Wan J, Xie W, Tao X, Yang P, Zhao L, Zhai Z, Wang C
Journal of translational internal medicine 2024 Sep;12(4):437-451

Altered mRNA transport and local translation in iNeurons with RNA binding protein knockdown.

Dargan R, Mikheenko A, Johnson NL, Packer B, Li Z, Craig EJ, Sarbanes SL, Bereda C, Mehta PR, Keuss M, Nalls MA, Qi YA, Weller CA, Fratta P, Ryan VH
bioRxiv : the preprint server for biology 2024 Sep 27;

RNA damage compartmentalization by DHX9 stress granules.

Zhou Y, Panhale A, Shvedunova M, Balan M, Gomez-Auli A, Holz H, Seyfferth J, Helmstädter M, Kayser S, Zhao Y, Erdogdu NU, Grzadzielewska I, Mittler G, Manke T, Akhtar A
Cell 2024 Mar 28;187(7):1701-1718.e28

Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human.

Huang W, Xiong T, Zhao Y, Heng J, Han G, Wang P, Zhao Z, Shi M, Li J, Wang J, Wu Y, Liu F, Xi JJ, Wang Y, Zhang QC
Nature genetics 2024 Jan;56(1):124-135

SINE-Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation.

He T, Peng J, Yang S, Liu D, Gao S, Zhu Y, Chai Z, Lee BC, Wei R, Wang J, Liu Z, Jin JX
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(2):e2307505

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility.

Wang X, Fan X, Zhang J, Wang F, Chen J, Wen Y, Wang L, Li T, Li H, Gu H, Zhang Y, Yuan S
Cell reports 2024 Feb 27;43(2):113769

DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation.

Gupta A, Bansal M, Ding J, Pandit M, Sudarshan S, Ding HF
bioRxiv : the preprint server for biology 2024 Dec 17;

M2 Macrophage-Derived Exosomal lncRNA MIR4435-2HG Promotes Progression of Infantile Hemangiomas by Targeting HNRNPA1.

Li Z, Cao Z, Li N, Wang L, Fu C, Huo R, Xu G, Tian C, Bi J
International journal of nanomedicine 2023;18:5943-5960

hnRNPA1 SUMOylation promotes cold hypersensitivity in chronic inflammatory pain by stabilizing TRPA1 mRNA.

Zhang Q, Weng W, Gu X, Xiang J, Yang Y, Zhu MX, Gu W, He Z, Li Y
Cell reports 2023 Nov 28;42(11):113401

Increase in hnRNPA1 Expression Suffices to Kill Motor Neurons in Transgenic Rats.

Huang B, Liu X, Zhang T, Wu Q, Huang C, Xia XG, Zhou H
International journal of molecular sciences 2023 Nov 11;24(22)

β-Hydroxybutyrate alleviates cartilage senescence through hnRNP A1-mediated up-regulation of PTEN.

Xia G, Wen Z, Zhang L, Huang J, Wang X, Liang C, Cui X, Cao X, Wu S
Experimental gerontology 2023 May;175:112140

A splicing silencer in SMN2 intron 6 is critical in spinal muscular atrophy.

Wang L, Ji Y, Chen Y, Bai J, Gao P, Feng P
Human molecular genetics 2023 Mar 6;32(6):971-983

N protein of PEDV plays chess game with host proteins by selective autophagy.

Zhai X, Kong N, Zhang Y, Song Y, Qin W, Yang X, Ye C, Ye M, Tong W, Liu C, Zheng H, Yu H, Zhang W, Yang X, Zhang G, Tong G, Shan T
Autophagy 2023 Aug;19(8):2338-2352

The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.

Li Y, Wang H, Wan J, Ma Q, Qi Y, Gu Z
Frontiers in oncology 2022;12:845931

Position-dependent effects of hnRNP A1/A2 in SMN1/2 exon7 splicing.

Qiu J, Qu R, Lin M, Xu J, Zhu Q, Zhang Z, Sun J
Biochimica et biophysica acta. Gene regulatory mechanisms 2022 Nov;1865(8):194875

Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.

Wu H, Du J, Li C, Li H, Guo H, Li Z
International journal of molecular sciences 2022 Mar 24;23(7)

Rescue of mis-splicing of a common SLC26A4 mutant associated with sensorineural hearing loss by antisense oligonucleotides.

Feng P, Xu Z, Chen J, Liu M, Zhao Y, Wang D, Han L, Wang L, Wan B, Xu X, Li D, Shu Y, Hua Y
Molecular therapy. Nucleic acids 2022 Jun 14;28:280-292

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Duan L, Zaepfel BL, Aksenova V, Dasso M, Rothstein JD, Kalab P, Hayes LR
Cell reports 2022 Jul 19;40(3):111106

Physiological tissue-specific and age-related reduction of mouse TDP-43 levels is regulated by epigenetic modifications.

Pacetti M, De Conti L, Marasco LE, Romano M, Rashid MM, Nubiè M, Baralle FE, Baralle M
Disease models & mechanisms 2022 Apr 1;15(4)

Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2.

Chang Y, Lu X, Qiu J
Turkish journal of biology = Turk biyoloji dergisi 2021;45(2):187-195

Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.

Jiang R, Su G, Chen X, Chen S, Li Q, Xie B, Zhao Y
Cancer letters 2021 Nov 28;521:308-321

Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.

Li WJ, He YH, Yang JJ, Hu GS, Lin YA, Ran T, Peng BL, Xie BL, Huang MF, Gao X, Huang HH, Zhu HH, Ye F, Liu W
Nature communications 2021 Mar 29;12(1):1946

Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.

Wu H, Cui M, Li C, Li H, Dai Y, Cui K, Li Z
Journal of agricultural and food chemistry 2021 Mar 17;69(10):3060-3068

An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.

Tran JR, Paulson DI, Moresco JJ, Adam SA, Yates JR, Goldman RD, Zheng Y
The Journal of cell biology 2021 Jan 4;220(1)

hnRNPM deficiency leads to cognitive deficits via disrupting synaptic plasticity.

Akinyemi AR, Li D, Zhang J, Liu Q
Neuroscience letters 2021 Apr 23;751:135824

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Sun L, Li P, Ju X, Rao J, Huang W, Ren L, Zhang S, Xiong T, Xu K, Zhou X, Gong M, Miska E, Ding Q, Wang J, Zhang QC
Cell 2021 Apr 1;184(7):1865-1883.e20

E3 ligase ZFP91 inhibits Hepatocellular Carcinoma Metabolism Reprogramming by regulating PKM splicing.

Chen D, Wang Y, Lu R, Jiang X, Chen X, Meng N, Chen M, Xie S, Yan GR
Theranostics 2020;10(19):8558-8572

Novel Non-Coding Transcript in NR4A3 Locus, LncNR4A3, Regulates RNA Processing Machinery Proteins and NR4A3 Expression.

Congrains A, Niemann FS, Duarte ADSS, Ferro KPV, Olalla-Saad ST
Frontiers in oncology 2020;10:569668

Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer.

Li H, Cui Z, Lv X, Li J, Gao M, Yang Z, Bi Y, Zhang Z, Wang S, Li S, Zhou B, Yin Z
Frontiers in oncology 2020;10:528520

Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates.

Aarum J, Cabrera CP, Jones TA, Rajendran S, Adiutori R, Giovannoni G, Barnes MR, Malaspina A, Sheer D
EMBO reports 2020 Oct 5;21(10):e49585

A novel miR-206/hnRNPA1/PKM2 axis reshapes the Warburg effect to suppress colon cancer growth.

Fu R, Yang P, Amin S, Li Z
Biochemical and biophysical research communications 2020 Oct 22;531(4):465-471

Transcriptome profiling reveals the high incidence of hnRNPA1 exon 8 inclusion in chronic myeloid leukemia.

Li SQ, Liu J, Zhang J, Wang XL, Chen D, Wang Y, Xu YM, Huang B, Lin J, Li J, Wang XZ
Journal of advanced research 2020 Jul;24:301-310

Zc3h13 Regulates Nuclear RNA m(6)A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Wen J, Lv R, Ma H, Shen H, He C, Wang J, Jiao F, Liu H, Yang P, Tan L, Lan F, Shi YG, He C, Shi Y, Diao J
Molecular cell 2018 Mar 15;69(6):1028-1038.e6

A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.

Huang JZ, Chen M, Chen D, Gao XC, Zhu S, Huang H, Hu M, Zhu H, Yan GR
Molecular cell 2017 Oct 5;68(1):171-184.e6

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.

Chen K, Huang C, Yuan J, Cheng H, Zhou R
Molecular biology and evolution 2014 Aug;31(8):2194-211

HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells.

Guo R, Li Y, Ning J, Sun D, Lin L, Liu X
PloS one 2013;8(4):e62729

Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.

Lu Q, Bai J, Zhang L, Liu J, Jiang Z, Michal JJ, He Q, Jiang P
Journal of proteome research 2012 May 4;11(5):2890-903

Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.

Markus MA, Marques FZ, Morris BJ
PloS one 2011;6(12):e28926

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 11176-1-AP(HNRNPA1 antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The HNRNPA1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human HNRNPA1. This antibody recognizes human,mouse,rat antigen. The HNRNPA1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 11176-1-AP.pdf

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