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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, HeLaS3, mouse brain, PC-12.
- Concentration
- 0.46 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP A1 Polyclonal Antibody (Product # PA5-28385). Sample (30 µg of whole cell lysate). A: H1299. B: HeLa S3. 12% SDS PAGE. hnRNP A1 Polyclonal Antibody (Product # PA5-28385) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNP A1 Polyclonal Antibody detects HNRNPA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.12% SDS-PAGE. HnRNP A1 Polyclonal Antibody (Product # PA5-28385) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP A1 Polyclonal Antibody (Product # PA5-28385). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. hnRNP A1 Polyclonal Antibody (Product # PA5-28385) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-hnRNP A1 Polyclonal Antibody, (Product # PA5-28385) and a 38 kDa band corresponding to hnRNP A1 was observed in the cell lines tested. An uncharacterized band (*) at ~60 kDa was also observed. Modified whole cell extracts (1%SDS) (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), SK-BR-3 (Lane 4), and A-431 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP A1 in paraformaldehyde-fixed HeLa cells using a hnRNP A1 polyclonal antibody (Product # PA5-28385) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of hnRNP A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with hnRNP A1 Rabbit Polyclonal Antibody (Product # PA5-28385) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nucleus localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNP A1 Polyclonal Antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. HnRNP A1 Polyclonal Antibody (Product # PA5-28385) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNP A1 Polyclonal Antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. HnRNP A1 Polyclonal Antibody (Product # PA5-28385) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
