Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- A00144-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD11b/ITGAM Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for CD11b detection. Tested with WB, IHC-F, ICC, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes.
Luo F, Li R, Zheng H, Xu Y, Yang L, Qu C, Hong G, Wan Q
Frontiers in bioengineering and biotechnology 2022;10:913080
Frontiers in bioengineering and biotechnology 2022;10:913080
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of HL-60 cells using anti-CD11b antibody (A00144-1). Overlay histogram showing HL-60 cells stained with A00144-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (A00144-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image