LS-C100021

antibody from LSBio

Targeting: CD14

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C100021

Provider

LSBio

Product name

CD14 Antibody (aa54-83) LS-C100021

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Storage

Store at 2-8°C for up to 2 weeks. For long term storage aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of CD14(arrow) using rabbit polyclonal CD14 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CD14 Antibody western blot of A549 cell line lysates (35 ug/lane). The CD14 antibody detected the CD14 protein (arrow).

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.