- MA1-23611 - Invitrogen Antibodies CD14 antibody

Submitted references Single-Cell RNA Transcriptomics Reveals the State of Hepatic Lymphatic Endothelial Cells in Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.

Zhang P, Li H, Zhou C, Liu K, Peng B, She X, Cheng K, Liu H, Ming Y
Journal of clinical medicine 2022 May 20;11(10)

Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.

Paupert J, Mansat-De Mas V, Demur C, Salles B, Muller C
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53

Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.

Paupert J, Mansat-De Mas V, Demur C, Salles B, Muller C
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53

Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection.

Harvey HA, Post DM, Apicella MA
Infection and immunity 2002 Oct;70(10):5808-15

Immortalization of human urethral epithelial cells: a model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection.

Harvey HA, Post DM, Apicella MA
Infection and immunity 2002 Oct;70(10):5808-15

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 scRNA-seq revealed that a large number of monocyte/macrophages infiltrated into ACLF liver . ( A ) The workflow of scRNA-seq analyses. NPCs isolated from 5 ACLF livers, 3 cirrhosis livers, and 2 HC livers were included in this study. ( B ) 26,200 cells from HC ( n = 2), cirrhosis ( n = 3), and ACLF ( n = 5) human livers were divided into 20 clusters (0-19), shown by tSNE plotting. ( C ) The representative marker genes of 20 subpopulations are presented with a heat map. Each column represents a different cell cluster and is arranged in the order of 0-19. ( D ) The classical marker genes of each cluster: CD14 and CD68 for monocyte/macrophages, CD3D for T cells, CD79A for B cells, NKG7 and KLRD1 for NK cells and T cells, ALB and VWF for epithelial tissue cells (including epithelial cell and endothelial cells), CXCR2 for neutrophils, WDFY4 for dendritic cells, COL3A1 for fibroblasts. ( E ) We re-annotated the 20 subpopulations according to their marker genes, and 11 classical clusters were annotated to facilitate the next analyses. ( F ) Cell immunofluorescence of CD14 and CD68 in isolated monocyte/macrophages from ACLF liver (200x field). ( G ) The proportion of 11 classical clusters among HCs, cirrhosis patients, and ACLF patients. ( H ) IHC staining for monocyte/macrophages (CD14) in the liver of HCs, cirrhosis patients, and ACLF patients (100x field). (** p < 0.01; bar = 200 mum).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 SPP1 secreted by infiltrating monocyte/macrophages induced LyEC death and impaired the ability of tube formation of LyECs in vitro . ( A ) Cell immunofluorescence of CD14 and SPP1 in isolated monocyte/macrophages from ACLF liver (100x and 200x field). ( B ) Representative IHC images of SPP1 in liver tissues among HCs, cirrhosis patients, and ACLF patients (100x and 200x field). ( C ) Flow cytometry showed a higher proportion of 7-AAD-positive LyECs after SPP1 treatment (200 ng/mL and 1000 ng/mL). ( D ) The tube formation assays of LyECs for CNTL and SPP1 treatment (200 ng/mL and 1000 ng/mL) (100x field). (* p < 0.05, ** p < 0.01; bar = 200 mum).

MA1-23611

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