- MA1-82074 - Invitrogen Antibodies CD14 antibody

Submitted references Neutrophils dominate in opsonic phagocytosis of P. falciparum blood-stage merozoites and protect against febrile malaria.

Garcia-Senosiain A, Kana IH, Singh S, Das MK, Dziegiel MH, Hertegonne S, Adu B, Theisen M
Communications biology 2021 Aug 19;4(1):984

Expression of co-stimulatory molecules CD80 and CD86 is altered in CD14 + HLA-DR + monocytes from patients with Chagas disease following induction by Trypanosoma cruzi recombinant antigens.

Soares AK, Neves PA, Cavalcanti MD, Marinho SM, Oliveira W Júnior, Souza JR, Lorena VM, Gomes YM
Revista da Sociedade Brasileira de Medicina Tropical 2016 Sep-Oct;49(5):632-636

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8

Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.

Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11

Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.

Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11

Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.

Giavedoni LD, Velasquillo MC, Parodi LM, Hubbard GB, Hodara VL
Journal of virology 2000 Feb;74(4):1648-57

Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.

Giavedoni LD, Velasquillo MC, Parodi LM, Hubbard GB, Hodara VL
Journal of virology 2000 Feb;74(4):1648-57

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Malarial antibodies induce merozoite-phagocytosis by neutrophils and monocytes. a Percentage of individual leukocytes in PBLs from Danish blood donors ( n = 10) was enumerated by flow cytometry. Horizontal lines represent median values. b From each donor, 6 x 10 4 PBLs were incubated with unopsonized merozoites (UOP), merozoites opsonized with a pool of nonimmune plasma (NIP), or with immune plasma (IP) for 30 min at 37 degC for phagocytosis to occur. Cells were stained with anti-CD14, CD16, CD45, and CD66b antibodies and analyzed by flow cytometry to enumerate ethidium bromide (EtBr)-positive cells. The mean number (no.) of neutrophils (blue) and monocytes (red), which ingested merozoites are shown in the upper panel, and the percent contribution of neutrophils and monocytes to overall PBL phagocytosis is shown in lower panels for the given opsonized/unopsonized condition. c Mean percentage of monocytes or neutrophils in PBL preparations that phagocytosed merozoites in the presence of NIP, IP, or UOP condition. The percent phagocytosis of IP-opsonized merozoites is also shown for cytochalasin D (Cyt D) treated PLBs. d Kinetics of IP-opsonized merozoite uptake by monocytes and neutrophils present in Cyt D pretreated or untreated PBLs. Phagocytosis was stopped at indicated time points, and percent phagocytosis by the neutrophils and monocytes is expressed as the mean of triplicates. Data are represented from one of the two independent assays. Results were obtained with
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Merozoite-phagocytosis by PBLs depends on the degree of antibody opsonization. Merozoites were opsonized with serial dilutions of individual plasma ( n = 21) from Ghanaian children a or a pool of the same plasma b and c and incubated with PBLs (6 x 10 4 per well) for 30 min at 37 degC for phagocytosis to occur. Cells were stained with anti-CD14, CD16, CD45, and CD66b antibodies and analyzed by flow cytometry to enumerate EtBr-positive cells. a Left y -axis shows the median number (no.) of EtBr-positive neutrophils (blue solid line) and monocytes (red solid line) present in PBLs in the presence of individual plasma samples ( n = 21). Right y -axis shows the median percentage of EtBr neutrophils (dotted blue line) and monocytes (dotted red line) in EtBr-positive PBL fraction in the presence of individual plasma samples ( n = 21). b Mean percent of EtBr-positive neutrophils and monocytes in EtBr-positive PBL fraction in the presence of pooled plasma is plotted against the amount of antibody deposited on the merozoite surface as quantified by the merozoite-immunofluorescence assay (IFA). c Mean percentage of EtBr-positive neutrophils and monocytes present in PBL preparations either untreated or pretreated with FcgammaR blockers in the presence of pooled plasma is plotted against merozoite-IFA. The experiments in b and c were performed in triplicates.
Conjugate: Green dye

MA1-82074

Antibody data