AMAb90898

antibody from Atlas Antibodies

Targeting: CD14

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb90898

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90898, RRID:AB_2665715

Product name

Anti-CD14

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

PCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVE
IHAGGLNLEPFLKRVDADADPRQYADTVKALRVRR
LTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKI
TGTMPPLPLEATGLALSSL


Epitope

Binds to an epitope located within the peptide sequence AVEVEIHAGGLNLEP as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL1638

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human liver and pancreas tissues using AMAb90898 antibody. Corresponding CD14 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong immunoreactivity in a subset of lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human liver shows strong immunoreactivity in sinusoids.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong positivity in germinal center.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows absence of immunoreactivity (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows moderate membranous positivity in Kupffer cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate membranous positivity, mainly in germinal center cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows moderate membranous positivity in lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.