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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Flow cytometry [2]
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- Product number
- MAB4597 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD14 and recombinant mouse CD14 is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
Abdelhamid L, Hussein H, Ghanem M, Eissa N
Research in veterinary science 2017 Oct;114:225-232
Research in veterinary science 2017 Oct;114:225-232
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CD14 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CD14 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells were stained with Mouse Anti-Porcine/Equine CD14 Monoclonal Antibody (Catalog # MAB4597, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
