Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-37291 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses.
Bhattacharjee P, Keyel PA
Scientific reports 2018 Apr 24;8(1):6458
Scientific reports 2018 Apr 24;8(1):6458
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody (4B4F12) [NBP2-37291] - Analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (4B4F12) [NBP2-37291] - Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.