Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- Q10064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (TuK4), Qdot™ 800
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10064: peak excitation 405 (488) nm/peak emission 800 nm; recommended filter 780/60 nm. Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Infrared dye
- Isotype
- IgG
- Antibody clone number
- TuK4
- Vial size
- 100 µL
- Storage
- 4° C, store in dark
Submitted references Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.
Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.
Anaphylatoxin C5a Regulates 6-Sulfo-LacNAc Dendritic Cell Function in Human through Crosstalk with Toll-Like Receptor-Induced CREB Signaling.
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Rouers A, Chng MHY, Lee B, Rajapakse MP, Kaur K, Toh YX, Sathiakumar D, Loy T, Thein TL, Lim VWX, Singhal A, Yeo TW, Leo YS, Vora KA, Casimiro D, Lim B, Tucker-Kellogg L, Rivino L, Newell EW, Fink K
Cell reports. Medicine 2021 May 18;2(5):100278
Cell reports. Medicine 2021 May 18;2(5):100278
Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
Kreutmair S, Unger S, Núñez NG, Ingelfinger F, Alberti C, De Feo D, Krishnarajah S, Kauffmann M, Friebel E, Babaei S, Gaborit B, Lutz M, Jurado NP, Malek NP, Goepel S, Rosenberger P, Häberle HA, Ayoub I, Al-Hajj S, Nilsson J, Claassen M, Liblau R, Martin-Blondel G, Bitzer M, Roquilly A, Becher B
Immunity 2021 Jul 13;54(7):1578-1593.e5
Immunity 2021 Jul 13;54(7):1578-1593.e5
The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.
Ong SM, Hadadi E, Dang TM, Yeap WH, Tan CT, Ng TP, Larbi A, Wong SC
Cell death & disease 2018 Feb 15;9(3):266
Cell death & disease 2018 Feb 15;9(3):266
Anaphylatoxin C5a Regulates 6-Sulfo-LacNAc Dendritic Cell Function in Human through Crosstalk with Toll-Like Receptor-Induced CREB Signaling.
Zaal A, Dieker M, Oudenampsen M, Turksma AW, Lissenberg-Thunnissen SN, Wouters D, van Ham SM, Ten Brinke A
Frontiers in immunology 2017;8:818
Frontiers in immunology 2017;8:818
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Deng N, Mosmann TR
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Aug;87(8):777-83
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Aug;87(8):777-83
Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.
Kobie JJ, Treanor JJ, Ritchlin CT
Immunological investigations 2014;43(6):606-15
Immunological investigations 2014;43(6):606-15
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
Li X, Miao H, Henn A, Topham DJ, Wu H, Zand MS, Mosmann TR
Vaccine 2012 Jun 29;30(31):4581-4
Vaccine 2012 Jun 29;30(31):4581-4
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Scheible K, Zhang G, Baer J, Azadniv M, Lambert K, Pryhuber G, Treanor JJ, Topham DJ
Vaccine 2011 Mar 3;29(11):2159-68
Vaccine 2011 Mar 3;29(11):2159-68
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Qdot® 800 anti-human CD14 conjugate 780/60 band pass filter
- Conjugate
- Infrared dye