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Flow cytometry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB3832-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD14 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08571- Isotype
- IgG
- Antibody clone number
- 134620
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells.
Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy.
Granzymes A and K differentially potentiate LPS-induced cytokine response.
Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47.
Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-β and TNF-α.
Mycobacterium tuberculosis infection and tissue factor expression in macrophages.
Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Odent Grigorescu G, Rosca AM, Preda MB, Tutuianu R, Simionescu M, Burlacu A
Journal of tissue engineering and regenerative medicine 2017 Nov;11(11):3241-3252
Journal of tissue engineering and regenerative medicine 2017 Nov;11(11):3241-3252
Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy.
Hisada Y, Auriemma AC, Alexander W, Ay C, Mackman N
Thrombosis research 2017 Feb;150:65-72
Thrombosis research 2017 Feb;150:65-72
Granzymes A and K differentially potentiate LPS-induced cytokine response.
Wensink AC, Kok HM, Meeldijk J, Fermie J, Froelich CJ, Hack CE, Bovenschen N
Cell death discovery 2016;2:16084
Cell death discovery 2016;2:16084
Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
Zhang YN, Vernooij F, Ibrahim I, Ooi S, Gijsberts CM, Schoneveld AH, Sen KW, den Ruijter HM, Timmers L, Richards AM, Jong CT, Mazlan I, Wang JW, Lam CS, de Kleijn DP
PloS one 2016;11(1):e0148073
PloS one 2016;11(1):e0148073
Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1β Production and Activation through CD47.
Stein EV, Miller TW, Ivins-O'Keefe K, Kaur S, Roberts DD
Scientific reports 2016 Jan 27;6:19684
Scientific reports 2016 Jan 27;6:19684
Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-β and TNF-α.
Huizinga R, van Rijs W, Bajramovic JJ, Kuijf ML, Laman JD, Samsom JN, Jacobs BC
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5636-45
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5636-45
Mycobacterium tuberculosis infection and tissue factor expression in macrophages.
Kothari H, Rao LV, Vankayalapati R, Pendurthi UR
PloS one 2012;7(9):e45700
PloS one 2012;7(9):e45700
Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):635-45
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):635-45
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ
Journal of hepatology 2009 Aug;51(2):322-32
Journal of hepatology 2009 Aug;51(2):322-32
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Zhao Z, McCloud B, Fleming R, Klempner MS
Biochemical and biophysical research communications 2007 Jun 29;358(2):528-33
Biochemical and biophysical research communications 2007 Jun 29;358(2):528-33
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
