MAB982

antibody from R&D Systems

Targeting: CD14

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

MAB982

Provider

R&D Systems

Product name

Mouse CD14 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P10810


Isotype

IgG

Antibody clone number

159010

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.

Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F
EMBO molecular medicine 2015 Feb;7(2):211-26

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Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14.

Ma CY, Chang WE, Shi GY, Chang BY, Cheng SE, Shih YT, Wu HL
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1905-15

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Syndecan-1 shedding facilitates the resolution of neutrophilic inflammation by removing sequestered CXC chemokines.

Hayashida K, Parks WC, Park PW
Blood 2009 Oct 1;114(14):3033-43

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A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.

Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 15;183(2):1291-300

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse CD14 by Western Blot. Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CD14 Monoclonal Antibody (Catalog # MAB982) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.