Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF982 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CD14 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q4FJP7
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sialic Acid within the Glycosylphosphatidylinositol Anchor Targets the Cellular Prion Protein to Synapses.
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Prostaglandin D2 mediates neuronal damage by amyloid-beta or prions which activates microglial cells.
Bate C, Nolan W, McHale-Owen H, Williams A
The Journal of biological chemistry 2016 Aug 12;291(33):17093-101
The Journal of biological chemistry 2016 Aug 12;291(33):17093-101
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Ingham V, Williams A, Bate C
Journal of neuroinflammation 2014 Jun 21;11:115
Journal of neuroinflammation 2014 Jun 21;11:115
Prostaglandin D2 mediates neuronal damage by amyloid-beta or prions which activates microglial cells.
Bate C, Kempster S, Williams A
Neuropharmacology 2006 Feb;50(2):229-37
Neuropharmacology 2006 Feb;50(2):229-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CD14 by Western Blot. Western blot shows lysate of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CD14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF982) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD14 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.