Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [5]
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Validation data
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- Product number
- NB100-77758 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77758, RRID:AB_1083332
- Product name
- Mouse Monoclonal CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Bovine, Canine, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Conversion of mesenchymal stem cells into a canine hepatocyte-like cells by Foxa1 and Hnf4a.
Comparative Molecular Life History of Spontaneous Canine and Human Gliomas.
Increased motility of mesenchymal stem cells is correlated with inhibition of stimulated peripheral blood mononuclear cells in vitro.
Oxidative status predicts quality in human mesenchymal stem cells.
Comparison of Acute Thrombogenicity for Metallic and Polymeric Bioabsorbable Scaffolds: Magmaris Versus Absorb in a Porcine Arteriovenous Shunt Model.
CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes.
Canine Mesenchymal Stem Cell Potential and the Importance of Dog Breed: Implication for Cell-Based Therapies.
In vitro cell motility as a potential mesenchymal stem cell marker for multipotency.
Role of natural killer cells in the pathogenesis of human acute graft-versus-host disease.
Role of natural killer cells in the pathogenesis of human acute graft-versus-host disease.
Acute graft-versus-host-like disease induced by transplantation of human activated natural killer cells into SCID mice.
Acute graft-versus-host-like disease induced by transplantation of human activated natural killer cells into SCID mice.
Treatment of acute cellular rejection with T10B9.1A-31 or OKT3 in renal allograft recipients.
Treatment of acute cellular rejection with T10B9.1A-31 or OKT3 in renal allograft recipients.
Nitta S, Kusakari Y, Yamada Y, Kubo T, Neo S, Igarashi H, Hisasue M
Regenerative therapy 2020 Jun;14:165-176
Regenerative therapy 2020 Jun;14:165-176
Comparative Molecular Life History of Spontaneous Canine and Human Gliomas.
Amin SB, Anderson KJ, Boudreau CE, Martinez-Ledesma E, Kocakavuk E, Johnson KC, Barthel FP, Varn FS, Kassab C, Ling X, Kim H, Barter M, Lau CC, Ngan CY, Chapman M, Koehler JW, Long JP, Miller AD, Miller CR, Porter BF, Rissi DR, Mazcko C, LeBlanc AK, Dickinson PJ, Packer RA, Taylor AR, Rossmeisl JH Jr, Woolard KD, Heimberger AB, Levine JM, Verhaak RGW
Cancer cell 2020 Feb 10;37(2):243-257.e7
Cancer cell 2020 Feb 10;37(2):243-257.e7
Increased motility of mesenchymal stem cells is correlated with inhibition of stimulated peripheral blood mononuclear cells in vitro.
Bertolo A, Pavlicek D, Gemperli A, Baur M, Pötzel T, Stoyanov J
Journal of stem cells & regenerative medicine 2017;13(2):62-74
Journal of stem cells & regenerative medicine 2017;13(2):62-74
Oxidative status predicts quality in human mesenchymal stem cells.
Bertolo A, Capossela S, Fränkl G, Baur M, Pötzel T, Stoyanov J
Stem cell research & therapy 2017 Jan 6;8(1):3
Stem cell research & therapy 2017 Jan 6;8(1):3
Comparison of Acute Thrombogenicity for Metallic and Polymeric Bioabsorbable Scaffolds: Magmaris Versus Absorb in a Porcine Arteriovenous Shunt Model.
Waksman R, Lipinski MJ, Acampado E, Cheng Q, Adams L, Torii S, Gai J, Torguson R, Hellinga DM, Westman PC, Joner M, Zumstein P, Kolodgie FD, Virmani R
Circulation. Cardiovascular interventions 2017 Aug;10(8)
Circulation. Cardiovascular interventions 2017 Aug;10(8)
CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes.
He Z, Riva M, Björk P, Swärd K, Mörgelin M, Leanderson T, Ivars F
PloS one 2016;11(5):e0156377
PloS one 2016;11(5):e0156377
Canine Mesenchymal Stem Cell Potential and the Importance of Dog Breed: Implication for Cell-Based Therapies.
Bertolo A, Steffen F, Malonzo-Marty C, Stoyanov J
Cell transplantation 2015;24(10):1969-80
Cell transplantation 2015;24(10):1969-80
In vitro cell motility as a potential mesenchymal stem cell marker for multipotency.
Bertolo A, Gemperli A, Gruber M, Gantenbein B, Baur M, Pötzel T, Stoyanov J
Stem cells translational medicine 2015 Jan;4(1):84-90
Stem cells translational medicine 2015 Jan;4(1):84-90
Role of natural killer cells in the pathogenesis of human acute graft-versus-host disease.
Rhoades JL, Cibull ML, Thompson JS, Henslee-Downey PJ, Jennings CD, Sinn HP, Brown SA, Eichhorn TR, Cave ML, Jezek DA
Transplantation 1993 Jul;56(1):113-20
Transplantation 1993 Jul;56(1):113-20
Role of natural killer cells in the pathogenesis of human acute graft-versus-host disease.
Rhoades JL, Cibull ML, Thompson JS, Henslee-Downey PJ, Jennings CD, Sinn HP, Brown SA, Eichhorn TR, Cave ML, Jezek DA
Transplantation 1993 Jul;56(1):113-20
Transplantation 1993 Jul;56(1):113-20
Acute graft-versus-host-like disease induced by transplantation of human activated natural killer cells into SCID mice.
Xun C, Brown SA, Jennings CD, Henslee-Downey PJ, Thompson JS
Transplantation 1993 Aug;56(2):409-17
Transplantation 1993 Aug;56(2):409-17
Acute graft-versus-host-like disease induced by transplantation of human activated natural killer cells into SCID mice.
Xun C, Brown SA, Jennings CD, Henslee-Downey PJ, Thompson JS
Transplantation 1993 Aug;56(2):409-17
Transplantation 1993 Aug;56(2):409-17
Treatment of acute cellular rejection with T10B9.1A-31 or OKT3 in renal allograft recipients.
Waid TH, Lucas BA, Thompson JS, Brown SA, Munch L, Prebeck RJ, Jezek D
Transplantation 1992 Jan;53(1):80-6
Transplantation 1992 Jan;53(1):80-6
Treatment of acute cellular rejection with T10B9.1A-31 or OKT3 in renal allograft recipients.
Waid TH, Lucas BA, Thompson JS, Brown SA, Munch L, Prebeck RJ, Jezek D
Transplantation 1992 Jan;53(1):80-6
Transplantation 1992 Jan;53(1):80-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Human peripheral blood monocytes stained with M5E2 APC
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.